BMRB Entry 4959

Title:
Solution structure of the epsin N-terminal homology (ENTH) domain of human epsin
Deposition date:
2001-02-21
Original release date:
2001-05-22
Authors:
Koshiba, Seizo; Kigawa, Takanori; Kikuchi, Akira; Yokoyama, Shigeyuki
Citation:

Citation: Koshiba, Seizo; Kigawa, Takanori; Kikuchi, Akira; Yokoyama, Shigeyuki. "Solution structure of the epsin N-terminal homology (ENTH) domain of human epsin"  J. Struct. Func. Genomics 2, 1-8 (2001).

Assembly members:

Assembly members:
epsin ENTH domain, polymer, 148 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX2T

Experimental source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX2T

Data sets:
Data typeCount
1H chemical shifts920
13C chemical shifts606
15N chemical shifts142

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1epsin ENTH domain1

Entities:

Entity 1, epsin ENTH domain 148 residues - Formula weight is not available

1   GLYSERSERARGMETSERTHRSERSERLEU
2   ARGARGGLNMETLYSASNILEVALHISASN
3   TYRSERGLUALAGLUILELYSVALARGGLU
4   ALATHRSERASNASPPROTRPGLYPROSER
5   SERSERLEUMETSERGLUILEALAASPLEU
6   THRTYRASNVALVALALAPHESERGLUILE
7   METSERMETILETRPLYSARGLEUASNASP
8   HISGLYLYSASNTRPARGHISVALTYRLYS
9   ALAMETTHRLEUMETGLUTYRLEUILELYS
10   THRGLYSERGLUARGVALSERGLNGLNCYS
11   LYSGLUASNMETTYRALAVALGLNTHRLEU
12   LYSASPPHEGLNTYRVALASPARGASPGLY
13   LYSASPGLNGLYVALASNVALARGGLULYS
14   ALALYSGLNLEUVALALALEULEUARGASP
15   GLUASPARGLEUARGGLUGLUARG

Samples:

sample1: epsin ENTH domain, [U-13C; U-15N], 1.0 mM; sodium phosphate 20 mM; sodium chloride 400 mM; dithiothreitol, [U-2H], 2 mM; sodium azide 0.01%; H2O 90%; D2O 10%

sample2: epsin ENTH domain, [U-15N], 1.0 mM; sodium phosphate 20 mM; sodium chloride 400 mM; dithiothreitol, [U-2H], 2 mM; sodium azide 0.01%; H2O 90%; D2O 10%

cond1: pH: 6.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 15N HSQCnot availablenot availablenot available
3D 15N NOESY-HSQCnot availablenot availablenot available
3D 15N TOCSY-HSQCnot availablenot availablenot available
3D HNHBnot availablenot availablenot available
2D 13C HSQCnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D HN(CO)CAnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HCACOnot availablenot availablenot available
3D CBCANHnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HBHA(CO)NHnot availablenot availablenot available
3D C(CO)NHnot availablenot availablenot available
3D H(CCO)NHnot availablenot availablenot available
3D HCCH-TOCSYnot availablenot availablenot available
3D HCCH-COSYnot availablenot availablenot available
3D 13C NOESY-HSQCnot availablenot availablenot available
4D 13C/15N NOESYnot availablenot availablenot available
4D 13C/13C NOESYnot availablenot availablenot available

Software:

NMRPipe - data processing

NMRView - peak assignments

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ BAB14041 BAG62253 BAI45694
GB AAC33823 AAD38326 AAH44651 AAH46962 AAH67206
REF NP_001033759 NP_001123543 NP_001123544 NP_001239383 NP_034277
SP O88339 Q80VP1 Q9Y6I3
TPG DAA19370
AlphaFold Q80VP1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks