BMRB Entry 4955

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4955

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Title:
NMR Structure of Cysteinyl-phosphorylated Enzyme IIB of the N,N'-diacetylchitobiose-specific Phosphoenolpyruvate-dependent Phosphotransferase System of Escherichia coli
Deposition date:
2001-02-07
Original release date:
2001-08-22
Authors:
Ab, Eiso; Schuurman-Wolters, Gea; Nijlant, Dieter; Dijkstra, Klaas; Saier, Milton; Robillard, George; Scheek, Ruud
Citation:

Citation: Ab, Eiso; Schuurman-Wolters, Gea; Nijlant, Dieter; Dijkstra, Klaas; Saier, Milton; Robillard, George; Scheek, Ruud. "NMR Structure of Cysteinyl-phosphorylated Enzyme IIB of the N,N'-diacetylchitobiose-specific Phosphoenolpyruvate-dependent Phosphotransferase System of Escherichia coli"  J. Mol. Biol. 308, 993-1009 (2001).
PubMed: 11352587

Assembly members:

Assembly members:
phospho-IIB-chitobiose, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E.coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJR-BLIIB

Entity Sequences (FASTA):

Entity Sequences (FASTA):
phospho-IIB-chitobiose: MEKKHIYLFXSAGMSTSLLV SKMRAQAEKYEVPVIIEAFP ETLAGEKGQNADVVLLGPQI AYMLPEIQRLLPNKPVEVID SLLYGKVDGLGVLKAAVAAI KKAAAN

Data sets:
Data typeCount
13C chemical shifts482
15N chemical shifts114
1H chemical shifts623

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1phospho-IIB-chitobiose1

Entities:

Entity 1, phospho-IIB-chitobiose 106 residues - Formula weight is not available

1   METGLULYSLYSHISILETYRLEUPHECSP
2   SERALAGLYMETSERTHRSERLEULEUVAL
3   SERLYSMETARGALAGLNALAGLULYSTYR
4   GLUVALPROVALILEILEGLUALAPHEPRO
5   GLUTHRLEUALAGLYGLULYSGLYGLNASN
6   ALAASPVALVALLEULEUGLYPROGLNILE
7   ALATYRMETLEUPROGLUILEGLNARGLEU
8   LEUPROASNLYSPROVALGLUVALILEASP
9   SERLEULEUTYRGLYLYSVALASPGLYLEU
10   GLYVALLEULYSALAALAVALALAALAILE
11   LYSLYSALAALAALAASN

Samples:

sample_one: phospho-IIB-chitobiose, [U-15N; U-13C], 4 mM; Enzyme I 23 uM; HPr 34 uM; IIAchb 20 uM; PEP 100 mM; Tris-Acetate buffer 100 mM; NaCl 100 mM; DTT 5 mM; MgCl2 4 mM; Glycerol, [U-2H], 1.5%; D2O 10%

Sample_conditions_one: pH: 7.0; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
NMR_applied_experimentsample_onenot availableSample_conditions_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
DBJ BAA15519 BAB35867 BAG77432 BAI25754 BAI30732
EMBL CAA37069 CAP76232 CAQ32212 CAQ98655 CAR03098
GB AAC74808 AAG56724 AAN43080 AAN80596 AAP16973
REF NP_288171 NP_310471 NP_416252 NP_707373 NP_754031
SP P69795 P69796 P69830
AlphaFold P69795 P69796 P69830

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks