BMRB Entry 4953
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4953
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Citation: Parac, Tatjana; Vogtherr, Martin; Maurer, Marcus; Pahl, Andreas; Rueterjans, Heinz; Griesinger, Christian; Fiebig, Klaus. "Letter to the Editor: Assignment of 1H, 13C and 15N resonances of the PPIase
domain of the trigger factor from Mycoplasma genitalium" J. Biomol. NMR 20, 193-194 (2001).
Assembly members:
Peptidyl-prolyl-cis/trans isomerase, polymer, 113 residues, Formula weight is not available
Natural source: Common Name: Mycoplasma genitalium Taxonomy ID: 2097 Superkingdom: Bacteria Kingdom: not available Genus/species: Mycoplasma genitalium
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Peptidyl-prolyl-cis/trans isomerase: MRGSHHHHHHGSEKLAKTKS
TMVDVSDKKLANGDIAIIDF
TGIVDNKKLASASAQNYELT
IGSNSFIKGFETGLIAMKVN
QKKTLALTFPSDYHVKELQS
KPVTFEVVLKAIK
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 416 |
| 15N chemical shifts | 105 |
| 1H chemical shifts | 640 |
| coupling constants | 76 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | trigger factor | 1 |
Entities:
Entity 1, trigger factor 113 residues - Formula weight is not available
| 1 | MET | ARG | GLY | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | GLY | SER | GLU | LYS | LEU | ALA | LYS | THR | LYS | SER | ||||
| 3 | THR | MET | VAL | ASP | VAL | SER | ASP | LYS | LYS | LEU | ||||
| 4 | ALA | ASN | GLY | ASP | ILE | ALA | ILE | ILE | ASP | PHE | ||||
| 5 | THR | GLY | ILE | VAL | ASP | ASN | LYS | LYS | LEU | ALA | ||||
| 6 | SER | ALA | SER | ALA | GLN | ASN | TYR | GLU | LEU | THR | ||||
| 7 | ILE | GLY | SER | ASN | SER | PHE | ILE | LYS | GLY | PHE | ||||
| 8 | GLU | THR | GLY | LEU | ILE | ALA | MET | LYS | VAL | ASN | ||||
| 9 | GLN | LYS | LYS | THR | LEU | ALA | LEU | THR | PHE | PRO | ||||
| 10 | SER | ASP | TYR | HIS | VAL | LYS | GLU | LEU | GLN | SER | ||||
| 11 | LYS | PRO | VAL | THR | PHE | GLU | VAL | VAL | LEU | LYS | ||||
| 12 | ALA | ILE | LYS |
Samples:
sample_1: Peptidyl-prolyl-cis/trans isomerase, [U-100% 15N], 2.0 mM; sodium phosphate 50 mM
sample_2: Peptidyl-prolyl-cis/trans isomerase, [U-100% 13C; U-100% 15N], 2.0 mM; sodium phosphate 50 mM
sample_3: Peptidyl-prolyl-cis/trans isomerase 2.0 mM; sodium phosphate 50 mM
Ex-cond_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | not available | not available | Ex-cond_1 |
| TOCSY | not available | not available | Ex-cond_1 |
| 15N-edited NOESY | not available | not available | Ex-cond_1 |
| 15N-edited TOCSY | not available | not available | Ex-cond_1 |
| 13C-edited NOESY | not available | not available | Ex-cond_1 |
| HNCO | not available | not available | Ex-cond_1 |
| HNCA | not available | not available | Ex-cond_1 |
| HNCACB | not available | not available | Ex-cond_1 |
| CBCA(CO)NH | not available | not available | Ex-cond_1 |
| HCCH-TOCSY | not available | not available | Ex-cond_1 |
| H(CC)(CO)NH-TOCSY | not available | not available | Ex-cond_1 |
| (H)CC(CO)NH-TOCSY | not available | not available | Ex-cond_1 |
| HNHA | not available | not available | Ex-cond_1 |
Software:
xwinnmr v2.6 - acquisition, processing
XEASY v1.3.14 - assignment
NMR spectrometers:
- Bruker Avance DRX 800 MHz
- Bruker Avance DRX 600 MHz
Related Database Links:
| PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks