BMRB Entry 4945

Name: VAM3P N-TERMINAL DOMAIN SOLUTION STRUCTURE
Published: 2001-08-22

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PDB ID: 1hs7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4945
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

VAM3P N-TERMINAL DOMAIN SOLUTION STRUCTURE

Deposition date:

2001-01-22

Original release date:

2001-08-22

Authors:

Dulubova, I.; Yamaguchi, T.; Wang, Y.; Sudhof, T.; Rizo, J.

Citation:

Citation: Dulubova, I.; Yamaguchi, T.; Wang, Y.; Sudhof, T.; Rizo, J.. "Vam3p Structure Reveals Conserved and Divergent Properties of Syntaxins" Nat. Struct. Biol. 8, 258-264 (2001).
PubMed: 11224573

Assembly members:

Assembly members:
single chain biopolimer, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGex-KT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
single chain biopolimer: GSSQQEPQFSTNQKTKELSN LIETFAEQSRVLEKECTKIG SKRDSKELRYKIETELIPNC TSVRDKIESNILIHQNGKLS ADFKNLKTKYQSLQQSYNQR KSLFPLKTPISPGTSKERKD IHP

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	869
13C chemical shifts	502
15N chemical shifts	126

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Vam3p N-terminal domain	1

Entities:

Entity 1, Vam3p N-terminal domain 123 residues - Formula weight is not available

1

GLY

SER

GLN

GLU

PRO

GLN

PHE

SER

2

THR

ASN

GLN

LYS

THR

LYS

GLU

LEU

SER

ASN

3

LEU

ILE

GLU

THR

PHE

ALA

GLU

GLN

SER

ARG

4

VAL

LEU

GLU

LYS

GLU

CYS

THR

LYS

ILE

GLY

5

SER

LYS

ARG

ASP

SER

LYS

GLU

LEU

ARG

TYR

6

LYS

ILE

GLU

THR

GLU

LEU

ILE

PRO

ASN

CYS

7

THR

SER

VAL

ARG

ASP

LYS

ILE

GLU

SER

ASN

8

ILE

LEU

ILE

HIS

GLN

ASN

GLY

LYS

LEU

SER

9

ALA

ASP

PHE

LYS

ASN

LEU

LYS

THR

LYS

TYR

10

GLN

SER

LEU

GLN

SER

TYR

ASN

GLN

ARG

11

LYS

SER

LEU

PHE

PRO

LEU

LYS

THR

PRO

ILE

12

SER

PRO

GLY

THR

SER

LYS

GLU

ARG

LYS

ASP

13

ILE

HIS

PRO

Samples:

sample_1: single chain biopolimer, [U-99% 15N], 0.9 mM; phosphate buffer 20 mM

sample_2: single chain biopolimer, [U-99% 13C; U-99% 15N], 0.85 mM; phosphate buffer 20 mM

sample_cond_1: pH: 6.0; temperature: 303 K; ionic strength: 20 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	not available	not available	not available
2D NOESY	not available	not available	not available
HNHA	not available	not available	not available
3D 13C-separated NOESY	not available	not available	not available

Software:

VNMR v6.1B - collection

NMRPipe v1.7 - processing

NMRView v4.1 - data analysis

CNS v0.9 - structure solution

NMR spectrometers:

Varian INOVA 500 MHz
Varian INOVA 600 MHz

PDB	1HS7
DBJ	GAA26424
EMBL	CAA64026 CAA99304 CAY86392
GB	AAC49737 AHY77403 EDN63969 EDV10703 EEU07936
REF	NP_014749
SP	Q12241
TPG	DAA10881
AlphaFold	Q12241