BMRB Entry 4942

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4942

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C Resonance Assignments and Secondary Structure of the Liver Ribonuclease from Bullfrog Rana catesbeiana

Deposition date:

2001-01-18

Original release date:

2002-04-03

Authors:

Su, Ning-Yuan; Liao, You-Di; Chang, Chi-Fon; Wanga, Iren; Chen, Chinpan

Citation:

Citation: Su, Ning-Yuan; Liao, You-Di; Chang, Chi-Fon; Wanga, Iren; Chen, Chinpan. "Letter to the Editor: 1H, 15N and 13C Resonance Assignments and Secondary Structure of the Liver Ribonuclease from Bullfrog Rana catesbeiana" J. Biomol. NMR 20, 189-190 (2001).

Assembly members:

Assembly members:
rRC-RNase L1[M(-1),Q1], polymer, 112 residues, 12531 Da.

Natural source:

Natural source: Common Name: bullfrog Taxonomy ID: 8400 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rana catesbeiana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-11d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
rRC-RNase L1[M(-1),Q1]: MQNWAKFKEKHITSTSSIDC NTIMDKAIYIVGGKCKERNT FIISSEDNVKAICSGVSPDR KELSTTSFKLNTCIRDSITP RPCPYHPSPDNNKICVKCEK QLPVHFVGIGKC

Data sets:

assigned_chemical_shifts
- CS_1

Data type	Count
13C chemical shifts	491
15N chemical shifts	116
1H chemical shifts	774

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	rRC-RNase L1	1

Entities:

Entity 1, rRC-RNase L1 112 residues - 12531 Da.

1

MET

GLN

ASN

TRP

ALA

LYS

PHE

LYS

GLU

LYS

2

HIS

ILE

THR

SER

THR

SER

ILE

ASP

CYS

3

ASN

THR

ILE

MET

ASP

LYS

ALA

ILE

TYR

ILE

4

VAL

GLY

LYS

CYS

LYS

GLU

ARG

ASN

THR

5

PHE

ILE

SER

GLU

ASP

ASN

VAL

LYS

6

ALA

ILE

CYS

SER

GLY

VAL

SER

PRO

ASP

ARG

7

LYS

GLU

LEU

SER

THR

SER

PHE

LYS

LEU

8

ASN

THR

CYS

ILE

ARG

ASP

SER

ILE

THR

PRO

9

ARG

PRO

CYS

PRO

TYR

HIS

PRO

SER

PRO

ASP

10

ASN

LYS

ILE

CYS

VAL

LYS

CYS

GLU

LYS

11

GLN

LEU

PRO

VAL

HIS

PHE

VAL

GLY

ILE

GLY

12

LYS

CYS

Samples:

sample_1: rRC-RNase L1[M(-1),Q1], [U-15N; U-13C], 1.6 mM

condition_1: pH: 3.5; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C HSQC	not available	not available	not available
2D 1H-15N HSQC	not available	not available	not available
3D CBCA(CO)NH	not available	not available	not available
3D HNCACB	not available	not available	not available
3D HBHA(CO)NH	not available	not available	not available
3D HNCO	not available	not available	not available
3D HN(CA)CO	not available	not available	not available
3D 15N NOESY-HSQC	not available	not available	not available
3D 15N TOCSY-HSQC	not available	not available	not available
3D HCCH-TOCSY	not available	not available	not available
3D C(CO)NH	not available	not available	not available

Software:

xwinnmr v2.6 - data processing

AURELIA - assignment

NMR spectrometers:

Bruker DRX 600 MHz

GenBank	AAG30414
SWISS-PROT	P14626