BMRB Entry 4941

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4941
MolProbity Validation Chart

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Title:
The Three-dimensional Structure of the C-terminal DNA-binding Domain of Human Ku70
Deposition date:
2001-01-17
Original release date:
2002-01-23
Authors:
Zhang, Ziming; Zhu, Lingyang; Lin, Donghai; Chen, F.; Chen, David; Chen, Yuan
Citation:

Citation: Zhang, Ziming; Zhu, Lingyang; Lin, Donghai; Chen, F.; Chen, David; Chen, Yuan. "The Three-dimensional Structure of the C-terminal DNA-binding Domain of Human Ku70"  J. Biol. Chem. 276, 38231-38236 (2001).
PubMed: 11457852

Assembly members:

Assembly members:
Ku70, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ku70: MGSSHHHHHHSSGLVPRGSH MASPEGKVTKRKHDNEGSGS KRPKVEYSEEELKTHISKGT LGKFTVPMLKEACRAYGLKS GLKKQELLEALTKHFQD

Data sets:
Data typeCount
13C chemical shifts288
15N chemical shifts86
1H chemical shifts600

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Ku70 CTD1

Entities:

Entity 1, Ku70 CTD 97 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METALASERPROGLUGLYLYSVALTHRLYS
4   ARGLYSHISASPASNGLUGLYSERGLYSER
5   LYSARGPROLYSVALGLUTYRSERGLUGLU
6   GLULEULYSTHRHISILESERLYSGLYTHR
7   LEUGLYLYSPHETHRVALPROMETLEULYS
8   GLUALACYSARGALATYRGLYLEULYSSER
9   GLYLEULYSLYSGLNGLULEULEUGLUALA
10   LEUTHRLYSHISPHEGLNASP

Samples:

sample_1: Ku70, [U-15N], 0.8 – 1.5 mM; phosphate buffer 100 mM; NaN3 0.03%; DTT 5 mM

sample_2: Ku70, [U-13C; U-15N], 0.8 – 1.5 mM; phosphate buffer 100 mM; NaN3 0.03%; DTT 5 mM

conditions_1: pH: 6.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
HNCACBnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HCCH_TOCSYnot availablenot availablenot available
15N-editednot availablenot availablenot available
TOCSY-HSQCnot availablenot availablenot available
DQFCOSYnot availablenot availablenot available
13C-editednot availablenot availablenot available
NOESYnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available
HBHA(CO)NHnot availablenot availablenot available

Software:

FELIX v98 -

NMR spectrometers:

  • Varin Unity-plus 500 MHz

Related Database Links:

PDB
DBJ BAE00743 BAE01402 BAG10952 BAG37986 BAG51575
EMBL CAG30378 CAG47015 CAH93208 CAK54520 CAK54819
GB AAA36155 AAA51733 AAA61177 AAB22381 AAH08343
REF NP_001126888 NP_001267434 NP_001271637 NP_001275905 NP_001275906
SP P12956
AlphaFold P12956

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks