BMRB Entry 4940
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4940
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Birlirakis, Nicolas; Briand, Loic; Pernollet, Jean-Claude; Guittet, Eric. "Letter to the Editor: 1H, 13C and 15N chemical shift assignment of the honeybee
pheromone carrier protein ASP1" J. Biomol. NMR 20, 183-184 (2001).
Assembly members:
Antennal Specific Protein 1, polymer, 119 residues, 13180 Da.
Natural source: Common Name: Honeybee Taxonomy ID: 7460 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Apis mellifera lingustica
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Antennal Specific Protein 1: APDWVPPEVFDLVAEDKARC
MSEHGTTQAQIDDVDKGNLV
NEPSITCYMYCLLEAFSLVD
DEANVDEDIMLGLLPDQLQE
RAQSVMGKCLPTSGSDNCNK
IYNLAKCVQESAPDVWFVI
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 386 |
| 15N chemical shifts | 101 |
| 1H chemical shifts | 688 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ASP1 | 1 |
Entities:
Entity 1, ASP1 119 residues - 13180 Da.
| 1 | ALA | PRO | ASP | TRP | VAL | PRO | PRO | GLU | VAL | PHE | ||||
| 2 | ASP | LEU | VAL | ALA | GLU | ASP | LYS | ALA | ARG | CYS | ||||
| 3 | MET | SER | GLU | HIS | GLY | THR | THR | GLN | ALA | GLN | ||||
| 4 | ILE | ASP | ASP | VAL | ASP | LYS | GLY | ASN | LEU | VAL | ||||
| 5 | ASN | GLU | PRO | SER | ILE | THR | CYS | TYR | MET | TYR | ||||
| 6 | CYS | LEU | LEU | GLU | ALA | PHE | SER | LEU | VAL | ASP | ||||
| 7 | ASP | GLU | ALA | ASN | VAL | ASP | GLU | ASP | ILE | MET | ||||
| 8 | LEU | GLY | LEU | LEU | PRO | ASP | GLN | LEU | GLN | GLU | ||||
| 9 | ARG | ALA | GLN | SER | VAL | MET | GLY | LYS | CYS | LEU | ||||
| 10 | PRO | THR | SER | GLY | SER | ASP | ASN | CYS | ASN | LYS | ||||
| 11 | ILE | TYR | ASN | LEU | ALA | LYS | CYS | VAL | GLN | GLU | ||||
| 12 | SER | ALA | PRO | ASP | VAL | TRP | PHE | VAL | ILE |
Samples:
sample_1: Antennal Specific Protein 11.0 – 1.5 mM
sample_2: Antennal Specific Protein 1, [U-95% 15N], 1.0 – 1.5 mM
sample_3: Antennal Specific Protein 1, [U-95% 13C; U-95% 15N], 1.0 – 1.5 mM
cond_set_1: pH: 5.6; temperature: 295 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| For DRX800: TXI x/y/z gradient probe | not available | not available | cond_set_1 |
| For DRX500: TXI z gradient cryoprobe | not available | not available | cond_set_1 |
Software:
XWINNMR (Bruker) v2.6 -
FELIX (MSI) v98 -
NMR spectrometers:
- Bruker DRX 800 MHz
- Bruker DRX 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks