BMRB Entry 4939

Title:
Solution Structures of Two CCHC Zinc Fingers from the FOG Family Protein U-shaped that Mediate Protein-Protein Interactions
Deposition date:
2001-01-13
Original release date:
2001-01-23
Authors:
Kowalski, K.; Mackay, J.
Citation:

Citation: Liew, C.; Kowalski, K.; Fox, A.; Newton, A.; Sharpe, B.; Crossley, M.; Mackay, J.. "Solution Structures of Two CCHC Zinc Fingers from the FOG Family Protein U-shaped that Mediate Protein-Protein Interactions"  Structure 8, 1157-1166 (2000).

Assembly members:

Assembly members:
U-shaped transcriptional cofactor, polymer, 36 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PGEX-2T

Experimental source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
U-shaped transcriptional cofactor: GSAAEVMKKYCSTCDISFNY VKTYLAHKQFYCKNKP

Data sets:
Data typeCount
1H chemical shifts210
15N chemical shifts34

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1U-shaped transcriptional cofactor1
2ZINC ION2

Entities:

Entity 1, U-shaped transcriptional cofactor 36 residues - Formula weight is not available

1   GLYSERALAALAGLUVALMETLYSLYSTYR
2   CYSSERTHRCYSASPILESERPHEASNTYR
3   VALLYSTHRTYRLEUALAHISLYSGLNPHE
4   TYRCYSLYSASNLYSPRO

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: U-shaped transcriptional cofactor 0.4 mM; ZnSO4 0.6 mM; TCEP 0.6 mM

sample_2: U-shaped transcriptional cofactor, [U-15N], 0.2 mM; ZnSO4 0.3 mM; TCEP 0.3 mM

sample_cond_1: pH: 5.0; temperature: 293 K; ionic strength: 0.6 mM; pressure: 1 atm

sample_cond_2: pH: 5.0; temperature: 293 K; ionic strength: 0.3 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available

Software:

XWINNMR v2.1 - data processing

XEASY v1.3.13 - data analysis

DYANA v1.5 - structure solution

CNS v0.5 - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

REF XP_002077649
GenBank EDX03234
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks