BMRB Entry 4935

Name: Backbone Assignments for Af19
Published: 2001-06-29

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PDB ID: 1kvn
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4935
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Assignments for Af19

Deposition date:

2000-12-30

Original release date:

2001-06-29

Authors:

Pakhomova, Olga; Cui, Yumin; Zwieb, Christian; Hinck, Andrew

Citation:

Citation: Pakhomova, Olga; Cui, Yumin; Zwieb, Christian; Hinck, Andrew. "Letter to the Editor: Sequence-specific 1H, 13C, and 15N signal assignments and secondary structure of Archaeoglobus fulgidus SRP19" J. Biomol. NMR 20, 187-188 (2001).

Assembly members:

Assembly members:
Archaeglobus fulgidus SRP19, polymer, 104 residues, 12372.6 Da.

Natural source:

Natural source: Common Name: Archaeoglobus fulgidus Taxonomy ID: 2234 Superkingdom: Archaea Kingdom: not available Genus/species: Archaeoglobus fulgidus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Novagen pET23d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Archaeglobus fulgidus SRP19: MKESVVWTVNLDSKKSRAEG RRIPRRFAVPNVKLHELVEA SKELGLKFRAEEKKYPKSWW EEGGRVVVEKRGTKTKLMIE LARKIAEIREQKREQKKDKK KKKK

Data sets:

assigned_chemical_shifts
- shifts1

Data type	Count
1H chemical shifts	687
13C chemical shifts	425
15N chemical shifts	90

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Af19 monomer	1

Entities:

Entity 1, Af19 monomer 104 residues - 12372.6 Da.

1

MET

LYS

GLU

SER

VAL

TRP

THR

VAL

ASN

2

LEU

ASP

SER

LYS

SER

ARG

ALA

GLU

GLY

3

ARG

ILE

PRO

ARG

PHE

ALA

VAL

PRO

4

ASN

VAL

LYS

LEU

HIS

GLU

LEU

VAL

GLU

ALA

5

SER

LYS

GLU

LEU

GLY

LEU

LYS

PHE

ARG

ALA

6

GLU

LYS

TYR

PRO

LYS

SER

TRP

7

GLU

GLY

ARG

VAL

GLU

LYS

8

ARG

GLY

THR

LYS

THR

LYS

LEU

MET

ILE

GLU

9

LEU

ALA

ARG

LYS

ILE

ALA

GLU

ILE

ARG

GLU

10

GLN

LYS

ARG

GLU

GLN

LYS

ASP

LYS

11

LYS

Samples:

sample1: Archaeglobus fulgidus SRP19, [U-99% 13C; U-98% 15N], 1.8 – 2.2 mM; NaCl 0.050 M; KH2PO4 0.025 M; D2O 99.88%

sample2: Archaeglobus fulgidus SRP19, [U-98% 15N], 1.8 – 2.2 mM; NaCl 0.050 M; KH2PO4 0.025 M; D2O 5%; H2O 95%

condition1: pH: 6.0; temperature: 300 K; ionic strength: 0.075 M

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H/15N HSQC	not available	not available	condition1
2D 1H/13C CT-HSQC	not available	not available	condition1
3D CBCA(CO)NH	not available	not available	condition1
3D HNCACB	not available	not available	condition1
3D CCONH	not available	not available	condition1
3D HBHA(CO)NH	not available	not available	condition1
3D HNCO	not available	not available	condition1
3D HN(CA)CO	not available	not available	condition1
3D HCCH-TOCY	not available	not available	condition1

Software:

nmrPipe v1.7 - processing, apodization, zero filling, fourier transformation

PIPP v4.3.1 - Analysis, peak picking

XEASY v1.3.12 - analysis, peak picking, sequential assignment

NMR spectrometers:

Bruker AMX2 500 MHz

PDB	1KVN 1KVV
GB	AAB89988 AIG98129
REF	NP_070086 WP_010878753
SP	O29010
AlphaFold	O29010 O29010