BMRB Entry 4929
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4929
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Wu, Hongwei; Maciejewski, Mark; Benashski, Sharon; Mullen, Gregory; King, Stephen. "Letter to the Editor:
1H, 15N and 13C resonance assignments for the Tctex1 dynein light chain
from Chlamydomonas flagella" J. Biomol. NMR 20, 89-90 (2001).
Assembly members:
Chlamydomonas Tctex1, polymer, 114 residues, 12668 Da.
Natural source: Common Name: Chlamydomonas reinhardtii Taxonomy ID: 3055 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Chlamydomonas reinhardtii
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Chlamydomonas Tctex1: MEGVDPAVEEAAFVADDVSN
IIKESIDAVLQNQQYSEAKV
SQWTSSCLEHCIKRLTALNK
PFKYVVTCIIMQKNGAGLHT
AASCWWDSTTDGSRTVRWEN
KSMYCICTVFGLAI
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 391 |
| 13C chemical shifts | 403 |
| 15N chemical shifts | 110 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tctex1 subunit 1 | 1 |
| 2 | Tctex1 subunit 2 | 1 |
Entities:
Entity 1, Tctex1 subunit 1 114 residues - 12668 Da.
| 1 | MET | GLU | GLY | VAL | ASP | PRO | ALA | VAL | GLU | GLU | ||||
| 2 | ALA | ALA | PHE | VAL | ALA | ASP | ASP | VAL | SER | ASN | ||||
| 3 | ILE | ILE | LYS | GLU | SER | ILE | ASP | ALA | VAL | LEU | ||||
| 4 | GLN | ASN | GLN | GLN | TYR | SER | GLU | ALA | LYS | VAL | ||||
| 5 | SER | GLN | TRP | THR | SER | SER | CYS | LEU | GLU | HIS | ||||
| 6 | CYS | ILE | LYS | ARG | LEU | THR | ALA | LEU | ASN | LYS | ||||
| 7 | PRO | PHE | LYS | TYR | VAL | VAL | THR | CYS | ILE | ILE | ||||
| 8 | MET | GLN | LYS | ASN | GLY | ALA | GLY | LEU | HIS | THR | ||||
| 9 | ALA | ALA | SER | CYS | TRP | TRP | ASP | SER | THR | THR | ||||
| 10 | ASP | GLY | SER | ARG | THR | VAL | ARG | TRP | GLU | ASN | ||||
| 11 | LYS | SER | MET | TYR | CYS | ILE | CYS | THR | VAL | PHE | ||||
| 12 | GLY | LEU | ALA | ILE |
Samples:
Tctex1_dimer: Chlamydomonas Tctex1, [U-97% 13C; U-99% 15N], 1.4 mM
condition_1: pH: 6.7; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA (6388 x 4400 x 1418 Hz, 512 x 55 x 30, 48 scans) | not available | not available | not available |
| HN(CO)CA (6388 x 4400 x 141 8 Hz, 512 x 55 x 30, 48 scans) | not available | not available | not available |
| HNCACB (6388 x 8000 x 1418 Hz, 512 x 50 x 32, 64 scans) | not available | not available | not available |
| CBCA(CO)NH (6388 x 8000 x 1418 Hz, 512 x 46 x 31, 64 scans) | not available | not available | not available |
| HNCO (6388 x 2200 x 1418 Hz, 512 x 48 x 29, 32 scans) | not available | not available | not available |
| (HACA)CO(CA)NH (6388 x 2200 x 1418 Hz, 512 x 48 x 28, 80 scans) | not available | not available | not available |
| HNHA (7654 x 7654 x 1700 Hz, 512 x 60 x 28, 48 scans) | not available | not available | not available |
| 3D 1H-15N NOESY-HSQC (7654 x 7654 x 1700 Hz, 512 x 96 x 32, 32 scans) | not available | not available | not available |
| C(CO)NH-TOCSY (6388 x 9000 x 1418 Hz, 512 x 96 x 28, 48 scans) | not available | not available | not available |
| HC(CO)NH-TOCSY (6388 x 6388 x 1418 Hz, 512 x 72 x 28, 64 scans) | not available | not available | not available |
| HACA(CO)CANH (6388 x 2200 x 1418 Hz, 512 x 48 x 28, 80 scans). | not available | not available | not available |
| 2D 1H-15N HSQC (6388 x 1418 Hz, 512 x 80, 16 scans) | not available | not available | not available |
| 2D 1H-13C HSQC (7654 x 12000 Hz, 512 x 400, 64 scans) | not available | not available | not available |
Software:
FELIX v97.0 - processing spectra
XEASY v1.3.13 - assignments
NMR spectrometers:
- Varian Inova 500 MHz
- Varian Inova 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks