BMRB Entry 4928

Title:
Structure of the PHD Zinc finger from human Williams-Beuren syndrome transcription factor
Deposition date:
2000-12-18
Original release date:
2001-01-19
Authors:
Pascual, Jaime; Martinez-Yamout, M.; Dyson, H. Jane; Wright, Peter
Citation:

Citation: Pascual, Jaime; Martinez-Yamout, M.; Dyson, H. Jane; Wright, Peter. "Structure of the PHD Zinc finger from human Williams-Beuren syndrome transcription factor"  J. Mol. Biol. 304, 723-729 (2000).

Assembly members:

Assembly members:
WSTF-PHD, polymer, 51 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Experimental source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
WSTF-PHD: ARCKVCRKKGEDDKLILCDE CNKAFHLFCLRPALYEVPDG EWQCPACQPAT

Data sets:
Data typeCount
1H chemical shifts340
13C chemical shifts202
15N chemical shifts49

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1wstf-phd1
2Zn-12
3Zn-22

Entities:

Entity 1, wstf-phd 51 residues - Formula weight is not available

1   ALAARGCYSLYSVALCYSARGLYSLYSGLY
2   GLUASPASPLYSLEUILELEUCYSASPGLU
3   CYSASNLYSALAPHEHISLEUPHECYSLEU
4   ARGPROALALEUTYRGLUVALPROASPGLY
5   GLUTRPGLNCYSPROALACYSGLNPROALA
6   THR

Entity 2, Zn-1 - Zn - 65.409 Da.

1   ZN

Samples:

sample: WSTF-PHD, [U-15N; U-13C], 1.0 mM

sample_conditions: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCOsamplenot availablesample_conditions
HNCACBsamplenot availablesample_conditions
CBCA(CO)NHsamplenot availablesample_conditions
H(C)CH-COSYsamplenot availablesample_conditions
H(CCO)NH-TOCSY (mixing-time, tmix= 16.5 ms)samplenot availablesample_conditions
C(CO)NH-TOCSY (tmix= 16.5 ms)samplenot availablesample_conditions
15N HSQC-TOCSY (tmix= 75 ms)samplenot availablesample_conditions
15N HSQC-NOESY (tmix= 80 ms)samplenot availablesample_conditions
13C HSQC-NOESY (tmix= 80 ms)samplenot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker DMX 750 MHz

Related Database Links:

SWISS-PROT Q9Z277 Q9UIG0
REF XP_001111145 XP_001111105 XP_001077467 NP_115784 NP_035844
GenBank EAW69679 AAD08676 AAD08675 AAD04720 AAC97879
DBJ BAC29862 BAA89210
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts