BMRB Entry 4924
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4924
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Ellis, K.; Tenenholz, T.; Gilly, W.; Blaustein, M.; Weber, D.. "Interactions of a toxin from the scorpion Tityus serrulatus with a cloned K+ channel from squid (SqKv1A)." Biochemistry 40, 5942-5953 (2001).
Assembly members:
Tityustoxin K-alpha, polymer, 37 residues, Formula weight is not available
Natural source: Common Name: Brazilian scorpion Taxonomy ID: 6887 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Tityus Serrulatus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PSR9
Entity Sequences (FASTA):
Tityustoxin K-alpha: VFINAKCRGSPECLPKCKEA
IGKAAGKCMNGKCKCYP
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 240 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tityustoxin K-alpha | 1 |
Entities:
Entity 1, Tityustoxin K-alpha 37 residues - Formula weight is not available
| 1 | VAL | PHE | ILE | ASN | ALA | LYS | CYS | ARG | GLY | SER | ||||
| 2 | PRO | GLU | CYS | LEU | PRO | LYS | CYS | LYS | GLU | ALA | ||||
| 3 | ILE | GLY | LYS | ALA | ALA | GLY | LYS | CYS | MET | ASN | ||||
| 4 | GLY | LYS | CYS | LYS | CYS | TYR | PRO |
Samples:
sample_1: Tityustoxin K-alpha 4.53 mM
sample_cond_1: ionic strength: 1.44 mM; pH: 3.50; pressure: 1 atm; temperature: 310.00 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | not available | not available | not available |
| TOCSY | not available | not available | not available |
| DQF-COSY | not available | not available | not available |
| ROESY | not available | not available | not available |
| P.E.COSY | not available | not available | not available |
Software:
X-PLOR v3.1 - REFINEMENT, STRUCTURE SOLUTION
NMR spectrometers:
- Bruker DMX 600 MHz