BMRB Entry 4918
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4918
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NMR-STAR v3 text file.
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Citation: Keizer, David; Kalsiak, Michal; Suh, Jeong-Yong; Crump, Matthew; Irvin, Randall; Sykes, Brian. "Letter to the Editor: Assignments of 1H and 15N resonances of the Pseudomonas
aeruginosa K122-4 pilin monomer" J. Biomol. NMR 19, 385-386 (2001).
Assembly members:
Pseudomonas Type IV pilin, polymer, 129 residues, 13105 Da.
Natural source: Common Name: not available Taxonomy ID: 287 Superkingdom: Eubacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Pseudomonas Type IV pilin: ALEGTEFARAQLSEAMTLAS
GLKTKVSDIFSQDGSCPANT
AATAGIEKDTDINGKYVAKV
TTGGTAAASGGCTIVATMKA
SDVATPLRGKTLTLTLGNAD
KGSYTWACTSNADNKYLPKT
CQTATTTTP
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 15N chemical shifts | 124 |
| 1H chemical shifts | 632 |
| coupling constants | 80 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | pilin monomer | 1 |
Entities:
Entity 1, pilin monomer 129 residues - 13105 Da.
| 1 | ALA | LEU | GLU | GLY | THR | GLU | PHE | ALA | ARG | ALA | ||||
| 2 | GLN | LEU | SER | GLU | ALA | MET | THR | LEU | ALA | SER | ||||
| 3 | GLY | LEU | LYS | THR | LYS | VAL | SER | ASP | ILE | PHE | ||||
| 4 | SER | GLN | ASP | GLY | SER | CYS | PRO | ALA | ASN | THR | ||||
| 5 | ALA | ALA | THR | ALA | GLY | ILE | GLU | LYS | ASP | THR | ||||
| 6 | ASP | ILE | ASN | GLY | LYS | TYR | VAL | ALA | LYS | VAL | ||||
| 7 | THR | THR | GLY | GLY | THR | ALA | ALA | ALA | SER | GLY | ||||
| 8 | GLY | CYS | THR | ILE | VAL | ALA | THR | MET | LYS | ALA | ||||
| 9 | SER | ASP | VAL | ALA | THR | PRO | LEU | ARG | GLY | LYS | ||||
| 10 | THR | LEU | THR | LEU | THR | LEU | GLY | ASN | ALA | ASP | ||||
| 11 | LYS | GLY | SER | TYR | THR | TRP | ALA | CYS | THR | SER | ||||
| 12 | ASN | ALA | ASP | ASN | LYS | TYR | LEU | PRO | LYS | THR | ||||
| 13 | CYS | GLN | THR | ALA | THR | THR | THR | THR | PRO |
Samples:
sample_1: Pseudomonas Type IV pilin 2.2 mM
Ex-con_1: pH: 5.0; temperature: 303 K
Ex-cond_2: pH: 5.05; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H DQFCOSY | not available | not available | not available |
| 2D 1H-1H TOCSY | not available | not available | not available |
| 2D 1H-1H NOESY | not available | not available | not available |
| 2D 1H-13C CT HSQC | not available | not available | not available |
| 2D 1H-15N HSQC | not available | not available | not available |
| 3D 1H-1H-15N NOESY | not available | not available | not available |
| 3D 1H-1H-15N TOCSY | not available | not available | not available |
| 3D HNHA | not available | not available | not available |
Software:
NMRPIPE v1.8 - Processing of Spectra
NMRView v4.1.2 - Analysis of spectra
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian UNITY 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks