BMRB Entry 4908

Title:
Assignment of 1H, 13C and 15N resonances of the a'-domain of ERp57
Deposition date:
2000-12-01
Original release date:
2001-11-14
Authors:
Silvennoinen, Laura; Karvonen, Paivi; Koivunen, Peppi; Myllyharju, Johanna; Kivirikko, Kari; Kilpelainen, Ilkka
Citation:

Citation: Silvennoinen, Laura; Karvonen, Paivi; Koivunen, Peppi; Myllyharju, Johanna; Kivirikko, Kari; Kilpelainen, Ilkka. "Letter to the Editor: Assignment of 1H, 13C and 15N Resonances of the a'-domain of ERp57"  J. Biomol. NMR 20, 385-386 (2001).
PubMed: 11563561

Assembly members:

Assembly members:
ERp57, polymer, 120 residues, 13576 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE-30

Data sets:
Data typeCount
1H chemical shifts398
13C chemical shifts312
15N chemical shifts95

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ERp57 a'-domain1

Entities:

Entity 1, ERp57 a'-domain 120 residues - 13576 Da.

1   SERASNASPGLYPROVALLYSVALVALVAL
2   ALAGLUASNPHEASPGLUILEVALASNASN
3   GLUASNLYSASPVALLEUILEGLUPHETYR
4   ALAPROTRPCYSGLYHISCYSLYSASNLEU
5   GLUPROLYSTYRLYSGLULEUGLYGLULYS
6   LEUSERLYSASPPROASNILEVALILEALA
7   LYSMETASPALATHRALAASNASPVALPRO
8   SERPROTYRGLUVALARGGLYPHEPROTHR
9   ILETYRPHESERPROALAASNLYSLYSLEU
10   ASNPROLYSLYSTYRGLUGLYGLYARGGLU
11   LEUSERASPPHEILESERTYRLEUGLNARG
12   GLUALATHRASNPROPROVALILEGLNGLU

Samples:

sample_1: ERp57, [U-15N], 1.4 mM

sample_2: ERp57, [U-13C; U-15N], 2.6 mM

EXP1: pH: 6.16; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N-HSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
CC(CO)NHnot availablenot availablenot available
15N-edited TOCSYnot availablenot availablenot available
15N-edited NOESYnot availablenot availablenot available
13C-edited NOESYnot availablenot availablenot available

Software:

VNMR -

Felix -

XEASY -

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 800 MHz

Related Database Links:

BMRB 11102
PDB
DBJ BAG56782
GB ABQ22414 ACA57910 EQB77541
REF XP_004056140

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks