BMRB Entry 4902
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4902
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Hong, Eunmi; Shin, Joon; Bang, Eunjung; Kim, Min-Hyung; Lee, Seung-Taek; Lee, Weontae. "Letter to the Editor:
Complete sequence-specific 1H, 13C and 15N resonance assignments of the
human PTK6 SH2 domain" J. Biomol. NMR 19, 291-292 (2001).
Assembly members:
SH2 domian of Human Protein Tyrosine Kinase6, polymer, 100 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
SH2 domian of Human Protein Tyrosine Kinase6: SEPWFFGCISRSEAVRRLQA
EGNATGAFLIRVSEKPSADY
VLSVRDTQAVRHYKIWRRAG
GRLHLNEAVSFLSLPELVNY
HRAQSLSHGLRLAAPCRKHE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 661 |
| 13C chemical shifts | 403 |
| 15N chemical shifts | 96 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH2 domain | 1 |
Entities:
Entity 1, SH2 domain 100 residues - Formula weight is not available
| 1 | SER | GLU | PRO | TRP | PHE | PHE | GLY | CYS | ILE | SER | |
| 2 | ARG | SER | GLU | ALA | VAL | ARG | ARG | LEU | GLN | ALA | |
| 3 | GLU | GLY | ASN | ALA | THR | GLY | ALA | PHE | LEU | ILE | |
| 4 | ARG | VAL | SER | GLU | LYS | PRO | SER | ALA | ASP | TYR | |
| 5 | VAL | LEU | SER | VAL | ARG | ASP | THR | GLN | ALA | VAL | |
| 6 | ARG | HIS | TYR | LYS | ILE | TRP | ARG | ARG | ALA | GLY | |
| 7 | GLY | ARG | LEU | HIS | LEU | ASN | GLU | ALA | VAL | SER | |
| 8 | PHE | LEU | SER | LEU | PRO | GLU | LEU | VAL | ASN | TYR | |
| 9 | HIS | ARG | ALA | GLN | SER | LEU | SER | HIS | GLY | LEU | |
| 10 | ARG | LEU | ALA | ALA | PRO | CYS | ARG | LYS | HIS | GLU |
Samples:
sample-1: SH2 domian of Human Protein Tyrosine Kinase6, [U-98% 13C; U-99.8% 15N], 0.5 – 1.0 mM
Ex-cond_1: pH: 6.5; temperature: 298 K; ionic strength: 0.15 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-15N TOCSY | not available | not available | not available |
| 1H-15N NOESY | not available | not available | not available |
| 1H-13C HSQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNHA | not available | not available | not available |
| HMQC-J | not available | not available | not available |
| HNCO | not available | not available | not available |
| HCACO | not available | not available | not available |
| HNCACO | not available | not available | not available |
| 1H-13C NOESY | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
Software:
nmrPipe - data processing
NMR spectrometers:
- Bruker DRX 500 MHz
- Varian INOVA 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAG37660 BAI45737 |
| EMBL | CAA55295 |
| GB | AAC34935 AAH35843 EAW75262 EAW75263 |
| REF | NP_005966 XP_002830575 XP_003280201 XP_003809959 XP_004062576 |
| SP | Q13882 |
| AlphaFold | Q13882 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks