BMRB Entry 4898

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4898

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assignment of 1H, 13C, 15N and 31P resonances of the FYVE domain in the complex with phosphatidylinositol 3-phosphate.

Deposition date:

2000-11-13

Original release date:

2005-06-02

Authors:

Kutateladze, Tatiana; Overduin, Michael

Citation:

Citation: Kutateladze, Tatiana; Capelluto, D.; Ferguson, C.; Cheever, M.; Kutateladze, A.; Prestwich, G.; Overduin, Michael. "Multivalent mechanism of membrane insertion by the FYVE domain" J. Biol. Chem. 279, 3050-3057 (2004).
PubMed: 14578346

Assembly members:

Assembly members:
Early Endosome Antigen 1, polymer, 86 residues, Formula weight is not available
1,2 Dibutanoyl-sn-glycero-3-phospho[1D-myo-inositol], non-polymer, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Early Endosome Antigen 1: AVQELGRENQSLQIKHTQAL NRKWAEDNEVQNCMACGKGF SVTVRRHHCRQCGNIFCAEC SAKNALTPSSKKPVRVCDAC FNDLQG

Data sets:

assigned_chemical_shifts
- shift_set_1
- shift_set_2

Data type	Count
1H chemical shifts	616
13C chemical shifts	383
15N chemical shifts	109
31P chemical shifts	2

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FYVE	1
2	PtdIns3P	2
3	Zn-1	3
4	Zn-2	3

Entities:

Entity 1, FYVE 86 residues - Formula weight is not available

1

ALA

VAL

GLN

GLU

LEU

GLY

ARG

GLU

ASN

GLN

2

SER

LEU

GLN

ILE

LYS

HIS

THR

GLN

ALA

LEU

3

ASN

ARG

LYS

TRP

ALA

GLU

ASP

ASN

GLU

VAL

4

GLN

ASN

CYS

MET

ALA

CYS

GLY

LYS

GLY

PHE

5

SER

VAL

THR

VAL

ARG

HIS

CYS

ARG

6

GLN

CYS

GLY

ASN

ILE

PHE

CYS

ALA

GLU

CYS

7

SER

ALA

LYS

ASN

ALA

LEU

THR

PRO

SER

8

LYS

PRO

VAL

ARG

VAL

CYS

ASP

ALA

CYS

9

PHE

ASN

ASP

LEU

GLN

GLY

Entity 2, PtdIns3P 1 residues - C17 H32 O16 P2 - Formula weight is not available

1

PTDINS3P

Entity 3, Zn-1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: Early Endosome Antigen 1, [U-15N], 2.0 mM; 1,2 Dibutanoyl-sn-glycero-3-phospho[1D-myo-inositol] 1.0 mM; ZINC (II) ION 2.0 mM

sample_2: Early Endosome Antigen 1, [U-15N; U-13C], 2.0 mM; 1,2 Dibutanoyl-sn-glycero-3-phospho[1D-myo-inositol] 1.0 mM; ZINC (II) ION 2.0 mM

Ex-cond_1: pH: 6.7; temperature: 298 K; ionic strength: 0.2 M

Experiments:

Name	Sample	Sample state	Sample conditions
2D DIPSI	not available	not available	not available
2D NOESY	not available	not available	not available
1H-15N HSQC	not available	not available	not available
1H-13C HSQC	not available	not available	not available
1H-15N NOESY	not available	not available	not available
1H-15N HMQCJ	not available	not available	not available
1H-13C NOESY	not available	not available	not available
H(CCO)NH TOCSY	not available	not available	not available
C(CO)NH TOCSY	not available	not available	not available
1H-31P HARTMANN-HAHN	not available	not available	not available
HNHA	not available	not available	not available
13C F1-filtered, F2-edited NOESY	not available	not available	not available

Software:

NMRPipe - data processing

PIPP - peak picking

NMR spectrometers:

Varian Inova 600 MHz
Varian Inova 500 MHz
Varian Mercury 400 MHz

REF	XP_001926390 XP_001510589 XP_001915836 NP_003557 XP_001104577
SWISS-PROT	Q15075
GenBank	AAI56546 EAW97471 AAA79121
EMBL	CAA55632
PDB	1HYJ 1JOC 1HYI
BMRB	4579