BMRB Entry 4897

Title:
Sequential assignment, secondary structure and binding site of the carbohydrate-binding domain of papg from Uropathogenic E.coli
Deposition date:
2000-11-08
Original release date:
2001-02-27
Authors:
Sung, Mei-an; Chen, Ho An; Matthews, Stephen
Citation:

Citation: Sung, Mei-an; Chen, Ho An; Matthews, Stephen. "Letter to the Editor: Sequential assignment and secondary structure of the triple-labelled carbohydrate-binding domain of papG from uropathogenic E. coli"  J. Biomol. NMR 19, 197-198 (2001).

Assembly members:

Assembly members:
papG, polymer, 198 residues, 22729.6 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts569
15N chemical shifts184
1H chemical shifts184

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1papG1

Entities:

Entity 1, papG 198 residues - 22729.6 Da.

1   TRPASNASNILEVALPHETYRSERLEUGLY
2   ASNVALASNSERTYRGLNGLYGLYASNVAL
3   VALILETHRGLNARGPROGLNPHEILETHR
4   SERTRPARGPROGLYILEALATHRVALTHR
5   TRPASNGLNCYSASNGLYPROGLUPHEALA
6   ASPGLYSERTRPALATYRTYRARGGLUTYR
7   ILEALATRPVALVALPHEPROLYSLYSVAL
8   METTHRGLNASNGLYTYRPROLEUPHEILE
9   GLUVALHISASNLYSGLYSERTRPSERGLU
10   GLUASNTHRGLYASPASNASPSERTYRPHE
11   PHELEULYSGLYTYRLYSTRPASPGLUARG
12   ALAPHEASPALAGLYASNLEUCYSGLNLYS
13   PROGLYGLUTHRTHRARGLEUTHRGLULYS
14   PHEASPASPILEILEPHELYSVALALALEU
15   PROALAASPLEUPROLEUGLYASPTYRSER
16   VALTHRILEPROTYRTHRSERGLYILEGLN
17   ARGHISPHEALASERTYRLEUGLYALAARG
18   PHELYSILEPROTYRASNVALALALYSTHR
19   LEUPROARGGLUASNGLUMETLEUPHELEU
20   PHELYSASNILEGLYGLYLEUGLU

Samples:

Sample_1: papG, [U-99% 13C; U-99% 15N; U-90% 2H], 1.0 mM

Ex-cond_1: ionic strength: 0.03 M; pH: 5.2; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
d-HNCAnot availablenot availablenot available
d-HN(CO)CAnot availablenot availablenot available
d-HNCOnot availablenot availablenot available
d-HN(CA)COnot availablenot availablenot available
d-HN(CA)CBnot availablenot availablenot available
d-HN(COCA)CBnot availablenot availablenot available
z-shielded gradient, triple-resonance probe was equippednot availablenot availablenot available

Software:

AURELIA v2.1.1 - sequence assignments

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

EMBL CAA43555 CAR04491 CCP97646
GB AAA24293 AAL67407 AAN82031 AAN83601 AAP48795
REF NP_755458 NP_757027 WP_000758683 WP_000758684 WP_000758685

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks