BMRB Entry 4896

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4896

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Sequential Assignment and Secondary Structure of the 14kDa chemotactic Protein CheY2 from Sinorhizobium meliloti

Deposition date:

2000-11-08

Original release date:

2001-04-26

Authors:

Riepl, Hubert; Scharf, Birgit; Schmitt, Ruediger; Kalbitzer, Hans Robert; Maure, Till

Citation:

Citation: Riepl, Hubert; Scharf, Birgit; Schmitt, Ruediger; Kalbitzer, Hans Robert; Maure, Till. "Letter to the Editor: Sequential Assignment and Secondary Structure of the 14 kDa Chemotactic Protein CheY2 from Sinorhizobium meliloti" J. Biomol. NMR 19, 287-288 (2001).
PubMed: 11330820

Assembly members:

Assembly members:
response-regulator protein CheY2, polymer, 129 residues, 14000 Da.

Natural source:

Natural source: Common Name: Sinorhizobium meliloti Taxonomy ID: 382 Superkingdom: Eubacteria Kingdom: not available Genus/species: Sinorhizobium meliloti

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTYB1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
response-regulator protein CheY2: MSLAEKIKVLIVDDQVTSRL LLGDALQQLGFKQITAAGDG EQGMKIMAQNPHHLVISDFN MPKMDGLGLLQAVRANPATK KAAFIILTAQGDRALVQKAA ALGANNVLAKPFTIEKMKAA IEAVFGALK

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	453
13C chemical shifts	344
15N chemical shifts	118

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CheY2	1

Entities:

Entity 1, CheY2 129 residues - 14000 Da.

1

MET

SER

LEU

ALA

GLU

LYS

ILE

LYS

VAL

LEU

2

ILE

VAL

ASP

GLN

VAL

THR

SER

ARG

LEU

3

LEU

GLY

ASP

ALA

LEU

GLN

LEU

GLY

4

PHE

LYS

GLN

ILE

THR

ALA

GLY

ASP

GLY

5

GLU

GLN

GLY

MET

LYS

ILE

MET

ALA

GLN

ASN

6

PRO

HIS

LEU

VAL

ILE

SER

ASP

PHE

ASN

7

MET

PRO

LYS

MET

ASP

GLY

LEU

GLY

LEU

8

GLN

ALA

VAL

ARG

ALA

ASN

PRO

ALA

THR

LYS

9

LYS

ALA

PHE

ILE

LEU

THR

ALA

GLN

10

GLY

ASP

ARG

ALA

LEU

VAL

GLN

LYS

ALA

11

ALA

LEU

GLY

ALA

ASN

VAL

LEU

ALA

LYS

12

PRO

PHE

THR

ILE

GLU

LYS

MET

LYS

ALA

13

ILE

GLU

ALA

VAL

PHE

GLY

ALA

LEU

LYS

Samples:

sample_1: response-regulator protein CheY2, [U-15N], 1.0 mM; potassium phosphate 20 mM; D2O 10%; magnesium chloride 5 mM

sample_2: response-regulator protein CheY2, [U-15N; U-13C], 1.0 mM; potassium phosphate 20 mM; D2O 98%; magnesium chloride 5 mM

Ex-cond_1: pH: 6.80; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-1H NOESY	not available	not available	not available
1H-15N HSQC	not available	not available	not available
1H-15N TOCSY-HSQC	not available	not available	not available
1H-15N NOESY-HSQC	not available	not available	not available
HNCO	not available	not available	not available
HCACO	not available	not available	not available
HNCA	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
HBHA(CO)NH	not available	not available	not available
HCCH-TOCSY	not available	not available	not available
1H-13C HSQC	not available	not available	not available
1H-13C NOESY-HSQC	not available	not available	not available

Software:

XWINNMR - data collection and processing

AURELIA - data analysis

NMR spectrometers:

Bruker DMX 600 MHz
Bruker DMX 800 MHz

PDB	1P6Q 1P6U
EMBL	CAC45215 CCE94800 CCM66304 CDH84541
GB	AAA86678 ABR59098 ACP24056 AEG03193 AEG52102
REF	NP_384749 WP_003529938 WP_011974449 WP_012706841 WP_018324154