BMRB Entry 4890

Name: 1H resonance assignments for the N-ter domain of the human TFIIH MAT1 subunit
Published: 2000-12-04

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PDB ID: 1g25
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4890
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H resonance assignments for the N-ter domain of the human TFIIH MAT1 subunit

Deposition date:

2000-11-02

Original release date:

2000-12-04

Authors:

Gervais, V.; Wasielewski, E.; Busso, D.; Poterszman, A.; Egly, J.M.; Thierry, J.C.; Kieffer, B.

Citation:

Citation: Gervais, V.; Busso, D.; Wasielewski, E.; Poterszman, A.; Egly, J.M.; Thierry, J.C.; Kieffer, B.. "Solution Structure of the N-terminal Domain of the Human TFIIH MAT1 Subunit: New Insights into the RING Finger Family " J. Biol. Chem. ., .-..

Assembly members:

Assembly members:
Menage a trois, polymer, 65 residues, 7296 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Menage a trois: MDDQGCPRCKTTKYRNPSLK LMVNVCGHTLCESCVDLLFV RGAGNCPECGTPLRKSNFRV QLFED

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	424

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MAT1	1
2	Zn(II)-1	2
3	Zn(II)-2	2

Entities:

Entity 1, MAT1 65 residues - 7296 Da.

1

MET

ASP

GLN

GLY

CYS

PRO

ARG

CYS

LYS

2

THR

LYS

TYR

ARG

ASN

PRO

SER

LEU

LYS

3

LEU

MET

VAL

ASN

VAL

CYS

GLY

HIS

THR

LEU

4

CYS

GLU

SER

CYS

VAL

ASP

LEU

PHE

VAL

5

ARG

GLY

ALA

GLY

ASN

CYS

PRO

GLU

CYS

GLY

6

THR

PRO

LEU

ARG

LYS

SER

ASN

PHE

ARG

VAL

7

GLN

LEU

PHE

GLU

ASP

Entity 2, Zn(II)-1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: Menage a trois 2 mM

MAT1_conditions: pH: 7.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	sample_1	not available	MAT1_conditions
DQF-COSY	sample_1	not available	MAT1_conditions
2D TOCSY	sample_1	not available	MAT1_conditions

Software:

X-PLOR v3.8 - structure solution, refinement

XWINNMR v1.3 - data collection, data processing

xeasy v1.3.13 - data analysis

NMR spectrometers:

Bruker DMX 750 MHz

SWISS-PROT	P51948 P51949
REF	NP_703202 XP_001096910 NP_002422 NP_032638 NP_001098879
GenBank	AAH89023 AAH00820 AAH78712 AAA91741 AAB05248
EMBL	CAA63356 CAG29332 CAA61112
DBJ	BAE34168 BAB25556 BAE33464 BAB25483
PDB	1G25