BMRB Entry 4889

Title:
Backbone 1H and 15N Chemical Shift Assignment of c-Src SH3 complexed with RLP2 ligand in high salt solution
Deposition date:
2000-10-30
Original release date:
2002-01-23
Authors:
Wang, Chunyu; Pawley, Norma; Nicholson, Linda
Citation:

Citation: Wang, Chunyu; Pawley, Norma; Nicholson, Linda. "The Role of Backbone Motions in Ligand Binding to the c-Src SH3 Domain"  J. Mol. Biol. 313, 873-887 (2001).
PubMed: 11697910

Assembly members:

Assembly members:
Src Homology 3 domain, polymer, 72 residues, Formula weight is not available
RLP2, polymer, 9 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts70
15N chemical shifts70

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1c-Src SH31
2RLP22

Entities:

Entity 1, c-Src SH3 72 residues - Formula weight is not available

1   ALAGLYGLYVALTHRTHRPHEVALALALEU
2   TYRASPTYRGLUSERARGTHRGLUTHRASP
3   LEUSERPHELYSLYSGLYGLUARGLEUGLN
4   ILEVALASNASNTHRGLUGLYASPTRPTRP
5   LEUALAHISSERLEUTHRTHRGLYGLNTHR
6   GLYTYRILEPROSERASNTYRVALALAPRO
7   SERASPSERILEGLNALAGLUGLYILEHIS
8   ARGASP

Entity 2, RLP2 9 residues - Formula weight is not available

1   ARGALALEUPROPROLEUPROARGTYR

Samples:

sample_1: Src Homology 3 domain, [U-15N], 1 mM; RLP2 4 eq; sodium phosphate 50 mM; sodium sulphate 200 mM; DTT 10 mM; pepstatin 5 mg/ml; EDTA 50 uM; benzamidine 10 ug/ml; chloramphenicol 50 uM

condition_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
HNCAnot availablenot availablenot available

Software:

NMRpipe -

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

SWISS-PROT P00526 P12931 P00524 P00525 P00523
REF NP_955616 NP_056888 NP_938033 NP_001020566 NP_005408
GenBank AAA49078 AAA60584 AAA42571 AAA42583 AAA42565
EMBL CAC34523 CAA36156 CAA37004 CAA23696 CAA33404
PDB
DBJ BAE26865
BMRB 4888

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks