BMRB Entry 4889
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4889
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NMR-STAR v3 text file.
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Citation: Wang, Chunyu; Pawley, Norma; Nicholson, Linda. "The Role of Backbone Motions in Ligand Binding to the c-Src SH3 Domain" J. Mol. Biol. 313, 873-887 (2001).
PubMed: 11697910
Assembly members:
Src Homology 3 domain, polymer, 72 residues, Formula weight is not available
RLP2, polymer, 9 residues, Formula weight is not available
Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Src Homology 3 domain: AGGVTTFVALYDYESRTETD
LSFKKGERLQIVNNTEGDWW
LAHSLTTGQTGYIPSNYVAP
SDSIQAEGIHRD
RLP2: RALPPLPRY
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 70 |
| 15N chemical shifts | 70 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | c-Src SH3 | 1 |
| 2 | RLP2 | 2 |
Entities:
Entity 1, c-Src SH3 72 residues - Formula weight is not available
| 1 | ALA | GLY | GLY | VAL | THR | THR | PHE | VAL | ALA | LEU | ||||
| 2 | TYR | ASP | TYR | GLU | SER | ARG | THR | GLU | THR | ASP | ||||
| 3 | LEU | SER | PHE | LYS | LYS | GLY | GLU | ARG | LEU | GLN | ||||
| 4 | ILE | VAL | ASN | ASN | THR | GLU | GLY | ASP | TRP | TRP | ||||
| 5 | LEU | ALA | HIS | SER | LEU | THR | THR | GLY | GLN | THR | ||||
| 6 | GLY | TYR | ILE | PRO | SER | ASN | TYR | VAL | ALA | PRO | ||||
| 7 | SER | ASP | SER | ILE | GLN | ALA | GLU | GLY | ILE | HIS | ||||
| 8 | ARG | ASP |
Entity 2, RLP2 9 residues - Formula weight is not available
| 1 | ARG | ALA | LEU | PRO | PRO | LEU | PRO | ARG | TYR |
Samples:
sample_1: Src Homology 3 domain, [U-15N], 1 mM; RLP2 4 eq; sodium phosphate 50 mM; sodium sulphate 200 mM; DTT 10 mM; pepstatin 5 mg/ml; EDTA 50 uM; benzamidine 10 ug/ml; chloramphenicol 50 uM
condition_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | not available | not available | not available |
| 1H-15N TOCSY | not available | not available | not available |
| HNCA | not available | not available | not available |
Software:
NMRpipe -
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks