BMRB Entry 4876

Name: 1H,13C,15N assignment of Ca2+-bound state of Canine Milk Lysozyme at 30deg
Published: 2001-04-30

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4876

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H,13C,15N assignment of Ca2+-bound state of Canine Milk Lysozyme at 30deg

Deposition date:

2000-10-24

Original release date:

2001-04-30

Authors:

Kobashigawa, Yoshihiro; Nitta, Katsutoshi; Tsuda, Sakae

Citation:

Citation: Kobashigawa, Yoshihiro; Miura, Kazunori; Demura, Makoto; Nemoto, Nobuaki; Koshiba, Takumi; Nitta, Katsutoshi; Tsuda, Sakae. "Letter to the Editor: Assignment of 1H, 13C, and 15N Resonances of Canine Milk Lysozyme" J. Biomol. NMR 19, 387-388 (2001).

Assembly members:

Assembly members:
canine milk lysozyme, polymer, 130 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: dog Taxonomy ID: 9615 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Canis familiaris

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet22(b)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
canine milk lysozyme: SKIFSKCELARKLKSMGMDG FHGYSLANWVCMAEYESNFN TQAFNGRNSNGSSDYGIFQL NSKWWCKSNSHSSANACNIM CSKFLDDNIDDDIACAKRVV KDPNGMSAWVAWVKHCKGKD LSKYLASCNL

Data sets:

assigned_chemical_shifts
- shift_set1

Data type	Count
13C chemical shifts	244
15N chemical shifts	125
1H chemical shifts	260

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	canine milk lysozyme	1
2	Ca	2

Entities:

Entity 1, canine milk lysozyme 130 residues - Formula weight is not available

1	SER	LYS	ILE	PHE	SER	LYS	CYS	GLU	LEU	ALA
2	ARG	LYS	LEU	LYS	SER	MET	GLY	MET	ASP	GLY
3	PHE	HIS	GLY	TYR	SER	LEU	ALA	ASN	TRP	VAL
4	CYS	MET	ALA	GLU	TYR	GLU	SER	ASN	PHE	ASN
5	THR	GLN	ALA	PHE	ASN	GLY	ARG	ASN	SER	ASN
6	GLY	SER	SER	ASP	TYR	GLY	ILE	PHE	GLN	LEU
7	ASN	SER	LYS	TRP	TRP	CYS	LYS	SER	ASN	SER
8	HIS	SER	SER	ALA	ASN	ALA	CYS	ASN	ILE	MET
9	CYS	SER	LYS	PHE	LEU	ASP	ASP	ASN	ILE	ASP
10	ASP	ASP	ILE	ALA	CYS	ALA	LYS	ARG	VAL	VAL
11	LYS	ASP	PRO	ASN	GLY	MET	SER	ALA	TRP	VAL
12	ALA	TRP	VAL	LYS	HIS	CYS	LYS	GLY	LYS	ASP
13	LEU	SER	LYS	TYR	LEU	ALA	SER	CYS	ASN	LEU

Entity 2, Ca - Ca - 40.078 Da.

1

CA

Samples:

sample1: canine milk lysozyme, [U-15N; U-13C], 1.0 mM

Ex-cond_1: pH: 4.5; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
experiment_1	sample1	not available	Ex-cond_1

Software:

No software information available

NMR spectrometers:

Varian Inova 500 MHz

BMRB	4883 4887
PDB	1EL1 1I56 1QQY 2CWI
GenBank	AAB31794
REF	XP_851313
SWISS-PROT	P81708

1	SER	LYS	ILE	PHE	SER	LYS	CYS	GLU	LEU	ALA
2	ARG	LYS	LEU	LYS	SER	MET	GLY	MET	ASP	GLY
3	PHE	HIS	GLY	TYR	SER	LEU	ALA	ASN	TRP	VAL
4	CYS	MET	ALA	GLU	TYR	GLU	SER	ASN	PHE	ASN
5	THR	GLN	ALA	PHE	ASN	GLY	ARG	ASN	SER	ASN
6	GLY	SER	SER	ASP	TYR	GLY	ILE	PHE	GLN	LEU
7	ASN	SER	LYS	TRP	TRP	CYS	LYS	SER	ASN	SER
8	HIS	SER	SER	ALA	ASN	ALA	CYS	ASN	ILE	MET
9	CYS	SER	LYS	PHE	LEU	ASP	ASP	ASN	ILE	ASP
10	ASP	ASP	ILE	ALA	CYS	ALA	LYS	ARG	VAL	VAL
11	LYS	ASP	PRO	ASN	GLY	MET	SER	ALA	TRP	VAL
12	ALA	TRP	VAL	LYS	HIS	CYS	LYS	GLY	LYS	ASP
13	LEU	SER	LYS	TYR	LEU	ALA	SER	CYS	ASN	LEU

1	SER	LYS	ILE	PHE	SER	LYS	CYS	GLU	LEU	ALA
2	ARG	LYS	LEU	LYS	SER	MET	GLY	MET	ASP	GLY
3	PHE	HIS	GLY	TYR	SER	LEU	ALA	ASN	TRP	VAL
4	CYS	MET	ALA	GLU	TYR	GLU	SER	ASN	PHE	ASN
5	THR	GLN	ALA	PHE	ASN	GLY	ARG	ASN	SER	ASN
6	GLY	SER	SER	ASP	TYR	GLY	ILE	PHE	GLN	LEU
7	ASN	SER	LYS	TRP	TRP	CYS	LYS	SER	ASN	SER
8	HIS	SER	SER	ALA	ASN	ALA	CYS	ASN	ILE	MET
9	CYS	SER	LYS	PHE	LEU	ASP	ASP	ASN	ILE	ASP
10	ASP	ASP	ILE	ALA	CYS	ALA	LYS	ARG	VAL	VAL
11	LYS	ASP	PRO	ASN	GLY	MET	SER	ALA	TRP	VAL
12	ALA	TRP	VAL	LYS	HIS	CYS	LYS	GLY	LYS	ASP
13	LEU	SER	LYS	TYR	LEU	ALA	SER	CYS	ASN	LEU