BMRB Entry 4875

Title:
1H NMR Analysis of the partly-folded non-native two-disulphide intermediates (30-51, 5-14) and (30-51, 5-38) in the folding pathway of bovine pancreatic trypsin inhibitor
Deposition date:
2000-10-23
Original release date:
2002-08-12
Authors:
van Mierlo, Carlo; Kemmink, Johan; Neuhaus, David; Darby, Nigel; Creighton, Thomas
Citation:

Citation: van Mierlo, Carlo; Kemmink, Johan; Neuhaus, David; Darby, Nigel; Creighton, Thomas. "1H NMR Analysis of the partly-folded non-native two-disulphide intermediates (30-51, 5-14) and (30-51, 5-38) in the folding pathway of bovine pancreatic trypsin inhibitor"  J. Mol. Biol. 235, 1044-1061 (1994).

Assembly members:

Assembly members:
(30-51, 5-14)Ser BPTI folding intermediate, polymer, 58 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
(30-51, 5-14)Ser BPTI folding intermediate: RPDFCLEPPYTGPCKARIIR YFYNAKAGLCQTFVYGGSRA KRNNFKSAEDCRRTSGGA

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts370

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1(30-51, 5-14) BPTI1

Entities:

Entity 1, (30-51, 5-14) BPTI 58 residues - Formula weight is not available

1   ARGPROASPPHECYSLEUGLUPROPROTYR
2   THRGLYPROCYSLYSALAARGILEILEARG
3   TYRPHETYRASNALALYSALAGLYLEUCYS
4   GLNTHRPHEVALTYRGLYGLYSERARGALA
5   LYSARGASNASNPHELYSSERALAGLUASP
6   CYSARGARGTHRSERGLYGLYALA

Samples:

sample_1: (30-51, 5-14)Ser BPTI folding intermediate1.5 – 3 mM; H2O 90%; D2O 10%

sample_2: (30-51, 5-14)Ser BPTI folding intermediate1.5 – 3 mM; D2O 100%

Ex-cond_1: pH: 4.6; temperature: 271 K

Experiments:

NameSampleSample stateSample conditions
DQ = double quantum coherence experimentnot availablenot availableEx-cond_1

Software:

UXNMR - data acquisition, data processing

NMR spectrometers:

  • Bruker AMX 500 MHz