BMRB Entry 4863

Name: SOLUTION STRUCTURE OF NUCLEOLIN RBD2
Published: 2001-11-14

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4863

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SOLUTION STRUCTURE OF NUCLEOLIN RBD2

Deposition date:

2000-10-17

Original release date:

2001-11-14

Authors:

Allain, F.; Gilbert, D.; Bouvet, P.; Feigon, J.

Citation:

Citation: Allain, F.; Gilbert, D.; Bouvet, P.; Feigon, J.. "Solution Strcuture of the two N-terminal RNA-binding Domains of Nucleolin and NMR Study of the Interaction with its RNA Target" J. Mol. Biol. 303, 227-241 (2000).
PubMed: 11023788

Assembly members:

Assembly members:
NUCLEOLIN RBD2, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source: Common Name: golden hamster Taxonomy ID: 10036 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mesocricetus auratus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NUCLEOLIN RBD2: SHMLEDPCTSKKVRAARTLL AKNLSFNITEDELKEVFEDA LEIRLVSQDGKSKGIAYIEF KSEADAEKNLEEKQGAEIDG RSVSLYYTGEKGGTRG

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	252
15N chemical shifts	95
1H chemical shifts	589

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NUCLEOLIN RBD2	1

Entities:

Entity 1, NUCLEOLIN RBD2 96 residues - Formula weight is not available

1

SER

HIS

MET

LEU

GLU

ASP

PRO

CYS

THR

SER

2

LYS

VAL

ARG

ALA

ARG

THR

LEU

3

ALA

LYS

ASN

LEU

SER

PHE

ASN

ILE

THR

GLU

4

ASP

GLU

LEU

LYS

GLU

VAL

PHE

GLU

ASP

ALA

5

LEU

GLU

ILE

ARG

LEU

VAL

SER

GLN

ASP

GLY

6

LYS

SER

LYS

GLY

ILE

ALA

TYR

ILE

GLU

PHE

7

LYS

SER

GLU

ALA

ASP

ALA

GLU

LYS

ASN

LEU

8

GLU

LYS

GLN

GLY

ALA

GLU

ILE

ASP

GLY

9

ARG

SER

VAL

SER

LEU

TYR

THR

GLY

GLU

10

LYS

GLY

THR

ARG

GLY

Samples:

sample_1: NUCLEOLIN RBD2, [U-15N; U-13C], 1 mM; potassium phosphate 50 mM; KCl 100 mM; DTT, [U-2H], 5 mM; H2O 90%; D2O 10%

sample_2: NUCLEOLIN RBD2, [U-15N; U-13C], 1 mM; potassium phosphate 50 mM; KCl 100 mM; DTT, [U-2H], 5 mM; D2O 100%

sample_cond_1: ionic strength: 150 mM; pH: 6.2; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	not available	not available	not available
3D 15N-separated NOESY	not available	not available	not available
2D NOESY	not available	not available	not available

Software:

X-PLOR v3.841 - refinement, structure solution

DYANA v1 - structure solution

NMR spectrometers:

Bruker DRX 500 MHz

PDB	1FJC
GB	EGW00584