BMRB Entry 4860

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4860

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N Chemical Shift Assignments for human lymphocyte specific kinase (Lck) unique and SH3 domain

Deposition date:

2000-10-17

Original release date:

2000-12-07

Authors:

Briese, Lars; Hoffmann, Silke; Friedrich, Ute; Biesinger, Brigitte; Willbold, Dieter

Citation:

Citation: Briese, Lars; Hoffmann, Silke; Friedrich, Ute; Biesinger, Brigitte; Willbold, Dieter. "Letter to the Editor: Sequence-specific 1H, 13C and 15N resonance assignments of lymphocyte specific kinase unique and SH3 domains" J. Biomol. NMR 19, 193-194 (2001).

Assembly members:

Assembly members:
lymphocyte specific kinase, polymer, 126 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pLCKU3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
lymphocyte specific kinase: GPLGSMGCGCSSHPEDDWME NIDVCENCHYPIVPLDGKGT LLIRNGSEVRDPLVTYEGSN PPASPLQDNLVIALHSYEPS HDGDLGFEKGEQLRILEQSG EWWKAQSLTTGQEGFIPFNF VAKANS

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	622
13C chemical shifts	436
15N chemical shifts	118

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Lck unique SH3	1

Entities:

Entity 1, Lck unique SH3 126 residues - Formula weight is not available

1

GLY

PRO

LEU

GLY

SER

MET

GLY

CYS

GLY

CYS

2

SER

HIS

PRO

GLU

ASP

TRP

MET

GLU

3

ASN

ILE

ASP

VAL

CYS

GLU

ASN

CYS

HIS

TYR

4

PRO

ILE

VAL

PRO

LEU

ASP

GLY

LYS

GLY

THR

5

LEU

ILE

ARG

ASN

GLY

SER

GLU

VAL

ARG

6

ASP

PRO

LEU

VAL

THR

TYR

GLU

GLY

SER

ASN

7

PRO

ALA

SER

PRO

LEU

GLN

ASP

ASN

LEU

8

VAL

ILE

ALA

LEU

HIS

SER

TYR

GLU

PRO

SER

9

HIS

ASP

GLY

ASP

LEU

GLY

PHE

GLU

LYS

GLY

10

GLU

GLN

LEU

ARG

ILE

LEU

GLU

GLN

SER

GLY

11

GLU

TRP

LYS

ALA

GLN

SER

LEU

THR

12

GLY

GLN

GLU

GLY

PHE

ILE

PRO

PHE

ASN

PHE

13

VAL

ALA

LYS

ALA

ASN

SER

Samples:

sample_1: lymphocyte specific kinase, [U-15N; U-13C], 1.6 mM; sodium acetate 20 mM; 2-mercaptoethanol 10 mM

Ex-cond_1: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
15N-HSQC	not available	not available	not available
13C-HSQC	not available	not available	not available
15N NOESY-HSQC	not available	not available	not available
13C NOESY-HSQC	not available	not available	not available
HNCACB	not available	not available	not available
CBCACONH	not available	not available	not available
HCCONH	not available	not available	not available
CCONH	not available	not available	not available
HNCO	not available	not available	not available
HCCH-COSY	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Varian Unity Inova 750 MHz

BMRB	5794
PDB	1LCK 4D8K
DBJ	BAG64189 BAI45973
EMBL	CAA28691 CAA31884 CAA32211 CAC44027
GB	AAA18225 AAA59502 AAC50287 AAF34794 AAH13200
PRF	2201317A
REF	NP_001036236 NP_005347 XP_002802373 XP_003276479 XP_003828198
SP	P06239
TPE	CAD55807
AlphaFold	P06239