BMRB Entry 4859

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4859

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Title:
HIGH RESOLUTION SOLUTION STRUCTURE OF THE PROTEIN PART OF CU7 METALLOTHIONEIN
Deposition date:
2000-10-16
Original release date:
2002-04-04
Authors:
Bertini, I.; Hartmann, H.; Klein, T.; Liu, G.; Luchinat, C.; Weser, U.
Citation:

Citation: Bertini, I.; Hartmann, H.; Klein, T.; Liu, G.; Luchinat, C.; Weser, U.. "High Resolution Solution Structure of the Protein Part of Cu7 Metallothionein"  Eur. J. Biochem. 267, 1008-1018 (2000).
PubMed: 10672009

Assembly members:

Assembly members:
METALLOTHIONEIN, polymer, 40 residues, Formula weight is not available
CU1, non-polymer, 63.546 Da.

Natural source:

Natural source:   Common Name: yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
METALLOTHIONEIN: QNEGHECQCQCGSCKNNEQC QKSCSCPTGCNSDDKCPCGN

Data sets:
Data typeCount
1H chemical shifts212

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1METALLOTHIONEIN1
2Cu-12
3Cu-22
4Cu-32
5Cu-42
6Cu-52
7Cu-62
8Cu-72

Entities:

Entity 1, METALLOTHIONEIN 40 residues - Formula weight is not available

1   GLNASNGLUGLYHISGLUCYSGLNCYSGLN
2   CYSGLYSERCYSLYSASNASNGLUGLNCYS
3   GLNLYSSERCYSSERCYSPROTHRGLYCYS
4   ASNSERASPASPLYSCYSPROCYSGLYASN

Entity 2, Cu-1 - Cu - 63.546 Da.

1   CU1

Samples:

sample_1: METALLOTHIONEIN 5 mM; phosphate 18 mM; 2-mercaptoethanol 0.03%; H2O 90%; D2O 10%

sample_cond_1: pH: 6.5; temperature: 283 K; ionic strength: 0.02 M

sample_cond_2: pH: 6.5; temperature: 298 K; ionic strength: 0.02 M

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablenot available
2D TOCSYsample_1not availablenot available

Software:

XWINNMR v3.1 - data analysis, data processing

DYANA v1.5 - structure solution

AMBER v5 - refinement

Xeasy v3.1 - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ GAA23770
EMBL CAA40884 CAY80056 CAY80058
GB AAA34541 AAB68382 AAB68384 AAS56843 AAT93096
REF NP_011920 NP_011922
SP P0CX80 P0CX81
TPG DAA06746 DAA06748
AlphaFold P0CX80 P0CX81