BMRB Entry 4858

Name: SOLUTION STRUCTURE OF NUCLEOLIN RBD1
Published: 2001-11-14

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4858

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

SOLUTION STRUCTURE OF NUCLEOLIN RBD1

Deposition date:

2000-10-16

Original release date:

2001-11-14

Authors:

Allain, F.; Gilbert, D.; Bouvet, P.; Feigon, J.

Citation:

Citation: Allain, F.; Gilbert, D.; Bouvet, P.; Feigon, J.. "Solution Strcuture of the two N-terminal RNA-binding Domains of Nucleolin and NMR Study of the Interaction with its RNA Target" J. Mol. Biol. 303, 227-241 (2000).
PubMed: 11023788

Assembly members:

Assembly members:
NUCLEOLIN RBD1, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source: Common Name: golden hamster Taxonomy ID: 10036 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mesocricetus auratus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: BL21(DE3)PLYSS

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NUCLEOLIN RBD1: GSHMLEDPVEGSESTTPFNL FIGNLNPNKSVAELKVAISE LFAKNDLAVVDVRTGTNRKF GYVDFESAEDLEKALELTGL KVFGNEIKLEKPKGRDGTRG C

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	309
15N chemical shifts	100
1H chemical shifts	698

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NUCLEOLIN RBD1	1

Entities:

Entity 1, NUCLEOLIN RBD1 101 residues - Formula weight is not available

1

GLY

SER

HIS

MET

LEU

GLU

ASP

PRO

VAL

GLU

2

GLY

SER

GLU

SER

THR

PRO

PHE

ASN

LEU

3

PHE

ILE

GLY

ASN

LEU

ASN

PRO

ASN

LYS

SER

4

VAL

ALA

GLU

LEU

LYS

VAL

ALA

ILE

SER

GLU

5

LEU

PHE

ALA

LYS

ASN

ASP

LEU

ALA

VAL

6

ASP

VAL

ARG

THR

GLY

THR

ASN

ARG

LYS

PHE

7

GLY

TYR

VAL

ASP

PHE

GLU

SER

ALA

GLU

ASP

8

LEU

GLU

LYS

ALA

LEU

GLU

LEU

THR

GLY

LEU

9

LYS

VAL

PHE

GLY

ASN

GLU

ILE

LYS

LEU

GLU

10

LYS

PRO

LYS

GLY

ARG

ASP

GLY

THR

ARG

GLY

11

CYS

Samples:

sample_1: NUCLEOLIN RBD1, [U-15N; U-13C], 1 mM; potasium phosphate 50 mM; KCl 100 mM; DTT, [U-2H], 5 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 150 mM; pH: 6.2; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	not available	not available	not available
3D 15N-separated NOESY	not available	not available	not available
2D NOESY	not available	not available	not available

Software:

X-PLOR v3.841 - refinement, structure solution

DYANA v1 - structure solution

NMR spectrometers:

Bruker DRX 600 MHz