BMRB Entry 4857

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4857

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Title:
Solution structure and dynamic character of the histidine-containing phosphotransfer domain of anaerobic sensor kinase ArcB from Escherichia coli
Deposition date:
2000-10-15
Original release date:
2015-09-02
Authors:
Ikegami, Takahisa; Okada, Terumasa; Ohki, Izuru; Hirayama, Junya; Mizuno, Takeshi; Shirakawa, Masahiro
Citation:

Citation: Ikegami, Takahisa; Okada, Terumasa; Ohki, Izuru; Hirayama, Junya; Mizuno, Takeshi; Shirakawa, Masahiro. "Solution structure and dynamic character of the histidine-containing phosphotransfer domain of anaerobic sensor kinase ArcB from Escherichia coli"  Biochemistry ., .-..

Assembly members:

Assembly members:
Histidine-containing phosphotransfer domain of E. coli anaerobic sensor kinase ArcB, polymer, 125 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: bacteria   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSU2DH

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Histidine-containing phosphotransfer domain of E. coli anaerobic sensor kinase ArcB: TTEENSKSEALLDIPMLEQY LELVGPKLITDGLAVFEKMM PGYVSVLESNLTAQDKKGIV EEGHKIKGAAGSVGLRHLQQ LGQQIQSPDLPAWEDNVGEW IEEMKEEWRHDVEVLKAWVA KATKK

Data sets:
Data typeCount
13C chemical shifts513
15N chemical shifts134
1H chemical shifts875
coupling constants96

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HPt-ArcB1

Entities:

Entity 1, HPt-ArcB 125 residues - Formula weight is not available

1   THRTHRGLUGLUASNSERLYSSERGLUALA
2   LEULEUASPILEPROMETLEUGLUGLNTYR
3   LEUGLULEUVALGLYPROLYSLEUILETHR
4   ASPGLYLEUALAVALPHEGLULYSMETMET
5   PROGLYTYRVALSERVALLEUGLUSERASN
6   LEUTHRALAGLNASPLYSLYSGLYILEVAL
7   GLUGLUGLYHISLYSILELYSGLYALAALA
8   GLYSERVALGLYLEUARGHISLEUGLNGLN
9   LEUGLYGLNGLNILEGLNSERPROASPLEU
10   PROALATRPGLUASPASNVALGLYGLUTRP
11   ILEGLUGLUMETLYSGLUGLUTRPARGHIS
12   ASPVALGLUVALLEULYSALATRPVALALA
13   LYSALATHRLYSLYS

Samples:

sample_1: Histidine-containing phosphotransfer domain of E. coli anaerobic sensor kinase ArcB, [U-99% 13C; U-99% 15N], 1.5 mM

Ex-cond_1: ionic strength: 0.1 M; pH: 6.5; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
CBCANHnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CA)HAnot availablenot availablenot available
HBHA(CBCACO)NHnot availablenot availablenot available
HBHA(CBCA)NHnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available
(H)C(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
15N-edited TOCSYnot availablenot availablenot available
(HB)CB(CGCD)HDnot availablenot availablenot available
(HB)CB(CGCDCE)HEnot availablenot availablenot available

Software:

Pipp v4.2.4 - peak picking

NMRPipe v1.7 - data processing

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ BAB37512 BAE77254
EMBL CAA37397
GenBank AAA58012 AAG58344 AAN44715 AAN82410 AAP18529
REF AP_003753 NP_289784 NP_312116 NP_709008 NP_755836
SWISS-PROT P0AEC3 P0AEC4 P58363

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks