BMRB Entry 4855

Title:
(14-38, 30-51) Double-disulphide intermediate in folding of Bovine Pancreatic Trypsin Inhibitor: A two-dimensional 1H nuclear magnetic resonance study
Deposition date:
2000-10-13
Original release date:
2002-08-12
Authors:
van Mierlo, Carlo; Darby, Nigel; Neuhaus, David; Creighton, Thomas
Citation:

Citation: van Mierlo, Carlo; Darby, Nigel; Neuhaus, David; Creighton, Thomas. "(14-38, 30-51) Double-disulphide intermediate in folding of Bovine Pancreatic Trypsin Inhibitor: A two-dimensional 1H nuclear magnetic resonance study"  J. Mol. Biol. 222, 353-371 (1991).

Assembly members:

Assembly members:
(14-38, 30-51)Ser BPTI folding intermediate, polymer, 58 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
(14-38, 30-51)Ser BPTI folding intermediate: RPDFSLEPPYTGPCKARIIR YFYNAKAGLCQTFVYGGCRA KRNNFKSAEDCRRTSGGA

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts401

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1(14-38, 30-51)Ser BPTI1

Entities:

Entity 1, (14-38, 30-51)Ser BPTI 58 residues - Formula weight is not available

1   ARGPROASPPHESERLEUGLUPROPROTYR
2   THRGLYPROCYSLYSALAARGILEILEARG
3   TYRPHETYRASNALALYSALAGLYLEUCYS
4   GLNTHRPHEVALTYRGLYGLYCYSARGALA
5   LYSARGASNASNPHELYSSERALAGLUASP
6   CYSARGARGTHRSERGLYGLYALA

Samples:

sample_1: (14-38, 30-51)Ser BPTI folding intermediate2 – 3 mM; H2O 90%; D2O 10%

sample_2: (14-38, 30-51)Ser BPTI folding intermediate2 – 3 mM; D2O 100%

Ex-cond_1: pH: 4.6; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablenot available
Double Quantum Coherencenot availablenot availablenot available
NOESYnot availablenot availablenot available
ROESYnot availablenot availablenot available
TOCSYnot availablenot availablenot available

Software:

UXNMR - data acquisition, data processing

NMR spectrometers:

  • Bruker AM 500 MHz
  • Bruker AMX 500 MHz