BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4853

Title: 1H Chemical Shift Assignment for the Major Conformer in Solution of the Alpha-like Scorpion Toxin LqhIII

Deposition date: 2000-10-11 Original release date: 2008-07-17

Authors: Krimm, I.; Trivelli, X.; Lancelin, J.

Citation: Krimm, I.; Trivelli, X.; Lancelin, J.. "A Cis-trans Isomerism of a Non-prolyl Peptide Bond in Lqh III Alpha-like Scorpion Toxin Revealed by Solution NMR"  J. Mol. Biol. ., .-..

Assembly members:
LQH III ALPHA-LIKE TOXIN, polymer, 68 residues, Formula weight is not available

Natural source:   Common Name: Hebraeus yellow scorpion   Taxonomy ID: 6884   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Leiurus quinquestriatus

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
LQH III ALPHA-LIKE TOXIN: VRDGYIAQPENCVYHCFPGS SGCDTLCKEKGGTSGHCGFK VGHGLACWCNALPDNVGIIV EGEKCHSX

Data sets:
Data typeCount
1H chemical shifts406

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1LqhIII1

Entities:

Entity 1, LqhIII 68 residues - Formula weight is not available

1   VALARGASPGLYTYRILEALAGLNPROGLU
2   ASNCYSVALTYRHISCYSPHEPROGLYSER
3   SERGLYCYSASPTHRLEUCYSLYSGLULYS
4   GLYGLYTHRSERGLYHISCYSGLYPHELYS
5   VALGLYHISGLYLEUALACYSTRPCYSASN
6   ALALEUPROASPASNVALGLYILEILEVAL
7   GLUGLYGLULYSCYSHISSERNH2

Samples:

sample_1: LQH III ALPHA-LIKE TOXIN 2 mM; phosphate 100 mM; H2O 90%; D2O 10%

sample_2: LQH III ALPHA-LIKE TOXIN 2 mM; phosphate 100 mM; D2O 100%

sample_cond_1: pH: 5.8; temperature: 311 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
2D TOCSYnot availablenot availablesample_cond_1
DQF-COSYnot availablenot availablesample_cond_1

Software:

GIFA v4.2 - data analysis

XWINNMR vNA - data collection

X-PLOR v3.851 - structure solution

NMR spectrometers:

  • Bruker Avance DRX 500 MHz

Related Database Links:

PDB
SP P56678