BMRB Entry 4851
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4851
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Slupsky, Carolyn; Desautels, Michel; Huebert, Terry; Zhao, Ruohong; Hemmingsen, Sean; Mcintosh, Lawrence. "Structure of Cdc4p, a contractile ring protein essential for cytokinesis
in Schizosaccharomyces pombe" J. Biol. Chem. 276, 5943-5951 (2001).
Assembly members:
EF-hand PROTEIN (CDC4P), polymer, 141 residues, Formula weight is not available
Natural source: Common Name: fission yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PT7-7
Entity Sequences (FASTA):
EF-hand PROTEIN (CDC4P): MSTDDSPYKQAFSLFDRHGT
GRIPKTSIGDLLRACGQNPT
LAEITEIESTLPAEVDMEQF
LQVLNRPNGFDMPGDPEEFV
KGFQVFDKDATGMIGVGELR
YVLTSLGEKLSNEEMDELLK
GVPVKDGMVNYHDFVQMILA
N
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 944 |
| 13C chemical shifts | 457 |
| 15N chemical shifts | 146 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CDC4p | 1 |
Entities:
Entity 1, CDC4p 141 residues - Formula weight is not available
| 1 | MET | SER | THR | ASP | ASP | SER | PRO | TYR | LYS | GLN | ||||
| 2 | ALA | PHE | SER | LEU | PHE | ASP | ARG | HIS | GLY | THR | ||||
| 3 | GLY | ARG | ILE | PRO | LYS | THR | SER | ILE | GLY | ASP | ||||
| 4 | LEU | LEU | ARG | ALA | CYS | GLY | GLN | ASN | PRO | THR | ||||
| 5 | LEU | ALA | GLU | ILE | THR | GLU | ILE | GLU | SER | THR | ||||
| 6 | LEU | PRO | ALA | GLU | VAL | ASP | MET | GLU | GLN | PHE | ||||
| 7 | LEU | GLN | VAL | LEU | ASN | ARG | PRO | ASN | GLY | PHE | ||||
| 8 | ASP | MET | PRO | GLY | ASP | PRO | GLU | GLU | PHE | VAL | ||||
| 9 | LYS | GLY | PHE | GLN | VAL | PHE | ASP | LYS | ASP | ALA | ||||
| 10 | THR | GLY | MET | ILE | GLY | VAL | GLY | GLU | LEU | ARG | ||||
| 11 | TYR | VAL | LEU | THR | SER | LEU | GLY | GLU | LYS | LEU | ||||
| 12 | SER | ASN | GLU | GLU | MET | ASP | GLU | LEU | LEU | LYS | ||||
| 13 | GLY | VAL | PRO | VAL | LYS | ASP | GLY | MET | VAL | ASN | ||||
| 14 | TYR | HIS | ASP | PHE | VAL | GLN | MET | ILE | LEU | ALA | ||||
| 15 | ASN |
Samples:
sample_1: EF-hand PROTEIN (CDC4P), [U-15N], 4.5 – 5.5 mM; KCl 100 mM
sample_2: EF-hand PROTEIN (CDC4P), [U-15N; U-13C], 4.5 – 5.5 mM; KCl 100 mM
sample_cond_1: pH: 6.5; temperature: 303 K; ionic strength: 100 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | not available | not available | not available |
| 3D CBCA(CO)NH | not available | not available | not available |
| 3D C(CO)NH | not available | not available | not available |
| 3D HCCH-TOCSY | not available | not available | not available |
| 3D 15N-TOCSY-HSQC | not available | not available | not available |
| 3D 15N-NOESY-HSQC | not available | not available | not available |
| 3D HNHB | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| 2D COSY | not available | not available | not available |
| 3D-HNHA | not available | not available | not available |
| 3D 13C/15N-NOESY-HSQC | not available | not available | not available |
| 4D 13C/13C-HMQC-NOESY-HMQC | not available | not available | not available |
Software:
X-PLOR v3.8 - refinement, structure solution
NMR spectrometers:
- Varian Unity 500 MHz
Related Database Links:
| PDB | |
| EMBL | CAB55175 |
| GB | AAA67467 |
| PRF | 2119321A |
| REF | NP_594947 |
| SP | Q09196 |
| AlphaFold | Q09196 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks