BMRB Entry 4844

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4844

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone HN, N , Ca, C' and Cb assignment of the 25 kDa peptide methionine sulfoxide reductase from Erwinia chrysanthemi
Deposition date:
2000-09-29
Original release date:
2001-05-17
Authors:
Beraud, Sabine; Chambost, Jean-Pierre; Bersch, Beate; Gans, Pierre; Barras, Frederic; Marion, Dominique
Citation:

Citation: Beraud, Sabine; Chambost, Jean-Pierre; Bersch, Beate; Gans, Pierre; Barras, Frederic; Marion, Dominique. "Letter to the Editor: Backbone HN, N, Ca, C? and Cb assignment of the 25 kDa peptide methionine sulfoxide reductase from Erwinia chrysanthemi"  J. Biomol. NMR 20, 97-98 (2001).

Assembly members:

Assembly members:
methionine sulfoxide reductase, polymer, 221 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 556   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Erwinia chrysanthemi

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
methionine sulfoxide reductase: VIANFDKTQPIGQSDALPGR TTPMPVARLHVVNQHSMTHV PDHMEVAIFAMGCFWGVERL FWQQPGVYSTASGYCGGYTP NPTYREVCTGKTGHAEAVRV VFDPAVVSYPQLLQLFWENH NPAQGMRQGNDIGTQYRSAI YTLTTEQETAAKESYQRFQQ AMRDAGNDHDITTEIQPAGP FYYAEDEHQQYLHKNPDGYC GLGGIGVCLPPQGLEHHHHH H

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts198
13C chemical shifts612
15N chemical shifts198

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1pmsr1

Entities:

Entity 1, pmsr 221 residues - Formula weight is not available

1   VALILEALAASNPHEASPLYSTHRGLNPRO
2   ILEGLYGLNSERASPALALEUPROGLYARG
3   THRTHRPROMETPROVALALAARGLEUHIS
4   VALVALASNGLNHISSERMETTHRHISVAL
5   PROASPHISMETGLUVALALAILEPHEALA
6   METGLYCYSPHETRPGLYVALGLUARGLEU
7   PHETRPGLNGLNPROGLYVALTYRSERTHR
8   ALASERGLYTYRCYSGLYGLYTYRTHRPRO
9   ASNPROTHRTYRARGGLUVALCYSTHRGLY
10   LYSTHRGLYHISALAGLUALAVALARGVAL
11   VALPHEASPPROALAVALVALSERTYRPRO
12   GLNLEULEUGLNLEUPHETRPGLUASNHIS
13   ASNPROALAGLNGLYMETARGGLNGLYASN
14   ASPILEGLYTHRGLNTYRARGSERALAILE
15   TYRTHRLEUTHRTHRGLUGLNGLUTHRALA
16   ALALYSGLUSERTYRGLNARGPHEGLNGLN
17   ALAMETARGASPALAGLYASNASPHISASP
18   ILETHRTHRGLUILEGLNPROALAGLYPRO
19   PHETYRTYRALAGLUASPGLUHISGLNGLN
20   TYRLEUHISLYSASNPROASPGLYTYRCYS
21   GLYLEUGLYGLYILEGLYVALCYSLEUPRO
22   PROGLNGLYLEUGLUHISHISHISHISHIS
23   HIS

Samples:

sample: methionine sulfoxide reductase, [U-70% 2H; U-13C; U-15N], 1 – 1.5 mM

condition: pH: 5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HN(COCA)CBnot availablenot availablenot available

Software:

FELIX -

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

EMBL CAA10143
GB ADM99860
REF WP_013319285 WP_038924815
SP Q9ZEQ8
AlphaFold Q9ZEQ8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks