BMRB Entry 4839

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4839

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Title:
Full assignments of RSVPR deltaLAM
Deposition date:
2000-09-25
Original release date:
2001-04-26
Authors:
Reinking, Jeffrey; Schatz, Gisela; Vogt, Volker; Nicholson, Linda
Citation:

Citation: Reinking, Jeffrey; Schatz, Gisela; Vogt, Volker; Nicholson, Linda. "Letter to the editor: 1H, 15N and 13C assignments of a monomeric N-terminal deletion mutant of the Rous sarcoma virus protease"  J. Biomol. NMR 19, 279-280 (2001).

Assembly members:

Assembly members:
Rous sarcoma virus protease, polymer, 121 residues, 13285 Da.

Natural source:

Natural source:   Common Name: Rous sarcoma virus   Taxonomy ID: 11886   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rous sarcoma virus protease: TMEHKDRPLVRVILTNTGSH PVKQRSVYITALLDSGADIT IISEEDWPTDWPVMEAANPQ IHGIGGGIPMRKSRDMIELG VINRDGSLERPLLLFPAVAM VRGSILGRDCLQGLGLRLTN L

Data sets:
Data typeCount
1H chemical shifts674
13C chemical shifts433
15N chemical shifts106

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RSVPR deltaLAM1

Entities:

Entity 1, RSVPR deltaLAM 121 residues - 13285 Da.

1   THRMETGLUHISLYSASPARGPROLEUVAL
2   ARGVALILELEUTHRASNTHRGLYSERHIS
3   PROVALLYSGLNARGSERVALTYRILETHR
4   ALALEULEUASPSERGLYALAASPILETHR
5   ILEILESERGLUGLUASPTRPPROTHRASP
6   TRPPROVALMETGLUALAALAASNPROGLN
7   ILEHISGLYILEGLYGLYGLYILEPROMET
8   ARGLYSSERARGASPMETILEGLULEUGLY
9   VALILEASNARGASPGLYSERLEUGLUARG
10   PROLEULEULEUPHEPROALAVALALAMET
11   VALARGGLYSERILELEUGLYARGASPCYS
12   LEUGLNGLYLEUGLYLEUARGLEUTHRASN
13   LEU

Samples:

15N_sample: Rous sarcoma virus protease, [U-15N], 3 mg/mL; Na phosphate 20 mM; NaCl 100 mM; glycerol 1%; urea 0.4 M; DTT 10 mM; AEBSF 0.25 mM; D2O 7%

13C_sample: Rous sarcoma virus protease, [U-13C], 3 mg/mL; Na phosphate 20 mM; NaCl 100 mM; glycerol 1%; urea 0.4 M; DTT 10 mM; AEBSF 0.25 mM; D2O 7%

13C_sample_in_D20: Rous sarcoma virus protease, [U-13C], 3 mg/mL; Na phosphate 20 mM; NaCl 100 mM; glycerol 1%; urea 0.4 M; DTT 10 mM; AEBSF 0.25 mM; D2O 100%

conditions_1: pH*: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
2D homonuclear NOESYnot availablenot availablenot available
2D homonuclear TOCSYnot availablenot availablenot available
15N TOCSYnot availablenot availablenot available
15N NOESYnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACB (optimized for B)not availablenot availablenot available
CCC-TOCSYnot availablenot availablenot available
HCC-TOCSYnot availablenot availablenot available
HNCOnot availablenot availablenot available
HCACOnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
13C-NOESYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available

Software:

nmrPipe -

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA01499 BAC78442 BAG16456 BAK64239 BAK64245
EMBL CAA24512 CAA31790 CAA32008 CAA36153 CAA48534
GB AAA19606 AAA19607 AAA46299 AAA46302 AAA46305
PRF 2019512A 2019512B
REF NP_056886 NP_056887 NP_955613 YP_004222727
SP O92954 O92956 P03322 P03354 P0C776
AlphaFold O92954 O92956 P03322 P03354 P0C776

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks