BMRB Entry 4837
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4837
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Brennan, L.; Turner, D.; Fareleira, P.; Santos, H.. "Solution Structure of Methylophilus methylotrophus Cytochrome C'': Insights into
the Structural Basis of Heme-ligand Detachment" J. Mol. Biol. 308, 353-365 (2001).
PubMed: 11327772
Assembly members:
CYTOCHROME C'', polymer, 124 residues, 15000 Da.
PROTOPORPHYRIN IX CONTAINING FE, non-polymer, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 17 Superkingdom: Eubacteria Kingdom: not available Genus/species: Methylophilus methylotrophus
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
CYTOCHROME C'': DVTNAEKLVYKYTNIAHSAN
PMYEAPSITDGKIFFNRKFK
TPSGKEAACASCHTNNPANV
GKNIVTGKEIPPLAPRVNTK
RFTDIDKVEDEFTKHCNDIL
GADCSPSEKANFIAYLLTET
KPTK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 885 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CYTOCHROME C'' | 1 |
| 2 | Heme C | 2 |
Entities:
Entity 1, CYTOCHROME C'' 124 residues - 15000 Da.
| 1 | ASP | VAL | THR | ASN | ALA | GLU | LYS | LEU | VAL | TYR | ||||
| 2 | LYS | TYR | THR | ASN | ILE | ALA | HIS | SER | ALA | ASN | ||||
| 3 | PRO | MET | TYR | GLU | ALA | PRO | SER | ILE | THR | ASP | ||||
| 4 | GLY | LYS | ILE | PHE | PHE | ASN | ARG | LYS | PHE | LYS | ||||
| 5 | THR | PRO | SER | GLY | LYS | GLU | ALA | ALA | CYS | ALA | ||||
| 6 | SER | CYS | HIS | THR | ASN | ASN | PRO | ALA | ASN | VAL | ||||
| 7 | GLY | LYS | ASN | ILE | VAL | THR | GLY | LYS | GLU | ILE | ||||
| 8 | PRO | PRO | LEU | ALA | PRO | ARG | VAL | ASN | THR | LYS | ||||
| 9 | ARG | PHE | THR | ASP | ILE | ASP | LYS | VAL | GLU | ASP | ||||
| 10 | GLU | PHE | THR | LYS | HIS | CYS | ASN | ASP | ILE | LEU | ||||
| 11 | GLY | ALA | ASP | CYS | SER | PRO | SER | GLU | LYS | ALA | ||||
| 12 | ASN | PHE | ILE | ALA | TYR | LEU | LEU | THR | GLU | THR | ||||
| 13 | LYS | PRO | THR | LYS |
Entity 2, Heme C 1 residues - C34 H32 N4 O4 FE1 - Formula weight is not available
| 1 | HEC_ox |
Samples:
sample_1: CYTOCHROME C'' 3.5 mM; ampicillin 70 uM; kanamicine 50 uM; chloramphenicol 50 uM
sample_cond_1: pH: 5.6; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D-1H-NOESY | not available | not available | not available |
| 2D-1H-TOCSY | not available | not available | not available |
| 2D-1H-COSY | not available | not available | not available |
| 3D-(15N,1H) TOCSY-HSQC | not available | not available | not available |
| 3D-(15N,1H) NOESY-HSQC | not available | not available | not available |
Software:
XEASY -
NMR spectrometers:
- Varian Unity Plus 750 MHz