BMRB Entry 4830

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4830

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
The Hairpin Structure of the (6)F1(1)F2(2)F2 Fragment from Human Fibronectin Enhances Gelatin Binding
Deposition date:
2000-09-14
Original release date:
2001-08-01
Authors:
Pickford, Andrew; Smith, Steven; Staunton, David; Boyd, Jonathan; Campbell, Iain
Citation:

Citation: Pickford, Andrew; Smith, Steven; Staunton, David; Boyd, Jonathan; Campbell, Iain. "The Hairpin Structure of the (6)F1(1)F2(2)F2 Fragment from Human Fibronectin Enhances Gelatin Binding"  EMBO J. 20, 1519-1529 (2001).
PubMed: 11285216

Assembly members:

Assembly members:
6f11f22f2, polymer, 160 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
6f11f22f2: YGHCVTDSGVVYSVGMQWLK TQGNKQMLCTCLGNGVSCQE TAVTQTYGGNSNGEPCVLPF TYNGRTFYSCTTEGRQDGHL WCSTTSNYEQDQKYSFCTDH TVLVQTRGGNSNGALCHFPF LYNNHNYTDCTSEGRRDNMK WCGTTQNYDADQKFGFCPMA

Data sets:
Data typeCount
1H chemical shifts994
15N chemical shifts165

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
16f11f22f21

Entities:

Entity 1, 6f11f22f2 160 residues - Formula weight is not available

1   TYRGLYHISCYSVALTHRASPSERGLYVAL
2   VALTYRSERVALGLYMETGLNTRPLEULYS
3   THRGLNGLYASNLYSGLNMETLEUCYSTHR
4   CYSLEUGLYASNGLYVALSERCYSGLNGLU
5   THRALAVALTHRGLNTHRTYRGLYGLYASN
6   SERASNGLYGLUPROCYSVALLEUPROPHE
7   THRTYRASNGLYARGTHRPHETYRSERCYS
8   THRTHRGLUGLYARGGLNASPGLYHISLEU
9   TRPCYSSERTHRTHRSERASNTYRGLUGLN
10   ASPGLNLYSTYRSERPHECYSTHRASPHIS
11   THRVALLEUVALGLNTHRARGGLYGLYASN
12   SERASNGLYALALEUCYSHISPHEPROPHE
13   LEUTYRASNASNHISASNTYRTHRASPCYS
14   THRSERGLUGLYARGARGASPASNMETLYS
15   TRPCYSGLYTHRTHRGLNASNTYRASPALA
16   ASPGLNLYSPHEGLYPHECYSPROMETALA

Samples:

sample_1: 6f11f22f2, [U-98% 15N], 2.0 mM

Ex-cond_1: pH: 4.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1not availableEx-cond_1
2D TOCSYsample_1not availableEx-cond_1
2D NOESY (60ms mixing time)sample_1not availableEx-cond_1
3D [1H-15N]sample_1not availableEx-cond_1
TOCSYsample_1not availableEx-cond_1
3D [1H-15N] NOESY (60ms mixing time)sample_1not availableEx-cond_1

Software:

Felix v2.3 - Data processing

NMRView - Assignment

NMR spectrometers:

  • . . 750 MHz

Related Database Links:

GenBank AAD00015
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks