BMRB Entry 4825

Name: 1H, 15N and 13C NMR Resonance Assignments of RC-RNase 2
Published: 2000-11-13

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PDB ID: 1m58
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4825
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C NMR Resonance Assignments of RC-RNase 2

Deposition date:

2000-09-11

Original release date:

2000-11-13

Authors:

Hsu, Chun-Hua; Chen, Lih-Woan; Liao, You-Di; Wu, Shih-Hsiung; Chen, Chinpan

Citation:

Citation: Hsu, Chun-Hua; Chen, Lih-Woan; Liao, You-Di; Wu, Shih-Hsiung; Chen, Chinpan. "1H, 15N and 13C resonance assignments and secondary structure determination of the RC-RNase 2 from oocytes of bullfrog Rana catesbeiana" J. Biomol. NMR 19, 87-88 (2001).

Assembly members:

Assembly members:
rRC-RNase2[M(-1),Q1], polymer, 106 residues, 12346 Da.

Natural source:

Natural source: Common Name: bullfrog Taxonomy ID: 8400 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rana catesbeiana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-11d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
rRC-RNase2[M(-1),Q1]: MQNWETFQKKHLTDTRDVKC DAEMKKALFDCKQKNTFIYA RPGRVQALCKNIIVSKNVLS TDEFYLSDCNRIKLPCHYKL KKSSNTICITCENKLPVHFV AVEECP

Data sets:

assigned_chemical_shifts
- Shift_set_1

Data type	Count
1H chemical shifts	768
13C chemical shifts	490
15N chemical shifts	112

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	rRC-RNase2[M(-1),Q1]	1

Entities:

Entity 1, rRC-RNase2[M(-1),Q1] 106 residues - 12346 Da.

1

MET

GLN

ASN

TRP

GLU

THR

PHE

GLN

LYS

2

HIS

LEU

THR

ASP

THR

ARG

ASP

VAL

LYS

CYS

3

ASP

ALA

GLU

MET

LYS

ALA

LEU

PHE

ASP

4

CYS

LYS

GLN

LYS

ASN

THR

PHE

ILE

TYR

ALA

5

ARG

PRO

GLY

ARG

VAL

GLN

ALA

LEU

CYS

LYS

6

ASN

ILE

VAL

SER

LYS

ASN

VAL

LEU

SER

7

THR

ASP

GLU

PHE

TYR

LEU

SER

ASP

CYS

ASN

8

ARG

ILE

LYS

LEU

PRO

CYS

HIS

TYR

LYS

LEU

9

LYS

SER

ASN

THR

ILE

CYS

ILE

THR

10

CYS

GLU

ASN

LYS

LEU

PRO

VAL

HIS

PHE

VAL

11

ALA

VAL

GLU

CYS

PRO

Samples:

sample_1: rRC-RNase2[M(-1),Q1], [U-15N; U-13C], 1.5 mM

cond-sample_1: pH: 3.5; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C HSQC	not available	not available	not available
2D 1H-15N HSQC	not available	not available	not available
3D CBCANH	not available	not available	not available
3D CBCA(CO)NH	not available	not available	not available
3D HBHA(CO)NH	not available	not available	not available
3D HNCO	not available	not available	not available
3D HN(CA)CO	not available	not available	not available
3D 15N NOESY-HSQC	not available	not available	not available
3D 15N TOCSY-HSQC	not available	not available	not available
3D HCCH-TOCSY	not available	not available	not available
3D C(CO)NH	not available	not available	not available

Software:

XWINNMR - data processing

AURELIA - assignment

NMR spectrometers:

Bruker DRX 600 MHz

PDB	1M58
GenBank	AAG31439