BMRB Entry 4820
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4820
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Citation: Liu, Aizhuo; Luginbuhl, Peter; Zerbe, Oliver; Ortenzi, Claudio; Luporini, Pierangelo; Wuthrich, Kurt. "Letter to the Editor:
NMR structure of the pheromone Er-22 from Euplotes raikovi" J. Biomol. NMR 19, 75-78 (2001).
Assembly members:
Pheromone Er-22, polymer, 37 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 5938 Superkingdom: Eukaryota Kingdom: not available Genus/species: Euplotes raikovi
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
Pheromone Er-22: DICDIAIAQCSLTLCQDCEN
TPICELAVKGSCPPPWS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 37 |
| 1H chemical shifts | 245 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Er-22 | 1 |
Entities:
Entity 1, Er-22 37 residues - Formula weight is not available
| 1 | ASP | ILE | CYS | ASP | ILE | ALA | ILE | ALA | GLN | CYS | ||||
| 2 | SER | LEU | THR | LEU | CYS | GLN | ASP | CYS | GLU | ASN | ||||
| 3 | THR | PRO | ILE | CYS | GLU | LEU | ALA | VAL | LYS | GLY | ||||
| 4 | SER | CYS | PRO | PRO | PRO | TRP | SER |
Samples:
H2O_sample: Pheromone Er-22 6.5 mM; H2O 90%; D2O 10%
D2O_sample: Pheromone Er-22 6.5 mM; D2O 100%
Exp-cond: pH: 5.0; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2QF-COSY | not available | not available | Exp-cond |
| clean-TOCSY | not available | not available | Exp-cond |
| 2Q-spectrum | not available | not available | Exp-cond |
| E.COSY | not available | not available | Exp-cond |
| NOESY | not available | not available | Exp-cond |
| 13C-1H HSQC | not available | not available | Exp-cond |
Software:
PROSA - spectral processing
XEASY - resonance assignment
DIANA - structure calculation
OPAL - restrained energy refinement
NMR spectrometers:
- Bruker AMX 600 MHz
- Varian Unity-plus 750 MHz