BMRB Entry 4814

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4814
MolProbity Validation Chart

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Title:
Complete 1H, 15N, and 13C assignments of an exchangeable apolipoprotein, Locusta migratoria apolipophorin III
Deposition date:
2000-08-24
Original release date:
2001-02-14
Authors:
Fan, Daping; Reese, Leslie; Ren, Xuefeng; Weers, Paul; Ryan, Robert; Wang, Jianjun
Citation:

Citation: Fan, Daping; Reese, Leslie; Ren, Xuefeng; Weers, Paul; Ryan, Robert; Wang, Jianjun. "Letter to the Editor: Complete 1H, 15N, and 13C assignments of an exchangeable apolipoprotein, Locusta migratoria apolipophorin III"  J. Biomol. NMR 19, 83-84 (2001).

Assembly members:

Assembly members:
apolipoprotein, polymer, 164 residues, 17501.92 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 7004   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Locusta migratoria

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
apolipoprotein: RPDAAGHVNIAEAVQQLNHT IVNAAHELHETLGLPTPDEA LNLLTEQANAFKTKIAEVTT SLKQEAEKHQGSVAEQLNAF ARNLNNSIHDAATSLNLQDQ LNSLQSALTNVGHQWQDIAT KTQASAQEAWAPVQSALQEA AEKTKEAAANLQNSIQSAVQ KPAN

Data sets:
Data typeCount
15N chemical shifts159
1H chemical shifts1098
13C chemical shifts324

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1exchangeable apolipoprotein1

Entities:

Entity 1, exchangeable apolipoprotein 164 residues - 17501.92 Da.

1   ARGPROASPALAALAGLYHISVALASNILE
2   ALAGLUALAVALGLNGLNLEUASNHISTHR
3   ILEVALASNALAALAHISGLULEUHISGLU
4   THRLEUGLYLEUPROTHRPROASPGLUALA
5   LEUASNLEULEUTHRGLUGLNALAASNALA
6   PHELYSTHRLYSILEALAGLUVALTHRTHR
7   SERLEULYSGLNGLUALAGLULYSHISGLN
8   GLYSERVALALAGLUGLNLEUASNALAPHE
9   ALAARGASNLEUASNASNSERILEHISASP
10   ALAALATHRSERLEUASNLEUGLNASPGLN
11   LEUASNSERLEUGLNSERALALEUTHRASN
12   VALGLYHISGLNTRPGLNASPILEALATHR
13   LYSTHRGLNALASERALAGLNGLUALATRP
14   ALAPROVALGLNSERALALEUGLNGLUALA
15   ALAGLULYSTHRLYSGLUALAALAALAASN
16   LEUGLNASNSERILEGLNSERALAVALGLN
17   LYSPROALAASN

Samples:

sample_1: apolipoprotein, [U-99% 13C; U-99% 15N], 0.6 – 1.0 mM

Normal-Cond: pH: 6.4; temperature: 303 K; ionic strength: 0.25 M

Experiments:

NameSampleSample stateSample conditions
A 5 mm triple-resonance probe with z-gradient. The above pulse sequences are from Dr.sample_1not availableNormal-Cond
Lewis Kay's laboratory at the University of Torontosample_1not availableNormal-Cond

Software:

NMRPIPE v1.7 - Spectral processing

PIPP v4.2.0 - Spectral assignment

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

PDB
GB AAA29282

Download HSQC peak lists in one of the following formats:
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