BMRB Entry 4795

Title:
Chemical Shift Assignments for Human D187N Gelsolin Domain 2
Deposition date:
2000-07-25
Original release date:
2000-11-13
Authors:
Kazmirski, Steven; Howard, Mark; Isaacson, Rivka; Fersht, Alan
Citation:

Citation: Kazmirski, Steven; Howard, Mark; Isaacson, Rivka; Fersht, Alan. "Elucidating the Mechanism of Familial Amyloidosis--Finnish Type: NMR Studies of Human Gelsolin Domain 2"  Proc. Natl. Acad. Sci. U.S.A. 97, 10706-10711 (2000).

Assembly members:

Assembly members:
Gelsolin Domain 2, polymer, 116 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapien

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts342
13C chemical shifts201
15N chemical shifts94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1D187N gelsolin domain 21

Entities:

Entity 1, D187N gelsolin domain 2 116 residues - Formula weight is not available

1   HISVALVALPROASNGLUVALVALVALGLN
2   ARGLEUPHEGLNVALLYSGLYARGARGVAL
3   VALARGALATHRGLUVALPROVALSERTRP
4   GLUSERPHEASNASNGLYASNCYSPHEILE
5   LEUASPLEUGLYASNASNILEHISGLNTRP
6   CYSGLYSERASNSERASNARGTYRGLUARG
7   LEULYSALATHRGLNVALSERLYSGLYILE
8   ARGASPASNGLUARGSERGLYARGALAARG
9   VALHISVALSERGLUGLUGLYTHRGLUPRO
10   GLUALAMETLEUGLNVALLEUGLYPROLYS
11   PROALALEUPROALAGLYTHRGLUASPTHR
12   ALALYSGLUASPALAALA

Samples:

sample_1: Gelsolin Domain 2, [U-13C; U-15N], 0.5 mM

Cond_1: pH: 7.2; temperature: 298 K; ionic strength: 20 mM

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker DRX 600 MHz

Related Database Links:

EMBL CAI14414 CAI14415
BMRB 4794
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks