BMRB Entry 4793
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4793
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Cort, J.; Yee, A.; Edwards, A.; Arrowsmith, C.; Kennedy, M.. "Structure-based Functional Classification of Hypothetical Protein MTH538 from
Methanobacterium thermoautotrophicum" J. Mol. Biol. 302, 189-203 (2000).
Assembly members:
HYPOTHETICAL PROTEIN MTH538, polymer, 132 residues, 14660 Da.
Natural source: Common Name: not available Taxonomy ID: 2166 Superkingdom: Archaea Kingdom: Euryarchaeota Genus/species: Methanobacterium thermoautotrophicum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET15B
Entity Sequences (FASTA):
HYPOTHETICAL PROTEIN MTH538: MGSSHHHHHHSSGLVPRGSH
MVTAEIRLYITEGEVEDYRV
FLERLEQSGLEWRPATPEDA
DAVIVLAGLWGTRRDEILGA
VDLARKSSKPIITVRPYGLE
NVPPELEAVSSEVVGWNPHC
IRDALEDALDVI
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 687 |
| 13C chemical shifts | 422 |
| 15N chemical shifts | 108 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HYPOTHETICAL PROTEIN MTH538 | 1 |
Entities:
Entity 1, HYPOTHETICAL PROTEIN MTH538 132 residues - 14660 Da.
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | VAL | THR | ALA | GLU | ILE | ARG | LEU | TYR | ILE | ||||
| 4 | THR | GLU | GLY | GLU | VAL | GLU | ASP | TYR | ARG | VAL | ||||
| 5 | PHE | LEU | GLU | ARG | LEU | GLU | GLN | SER | GLY | LEU | ||||
| 6 | GLU | TRP | ARG | PRO | ALA | THR | PRO | GLU | ASP | ALA | ||||
| 7 | ASP | ALA | VAL | ILE | VAL | LEU | ALA | GLY | LEU | TRP | ||||
| 8 | GLY | THR | ARG | ARG | ASP | GLU | ILE | LEU | GLY | ALA | ||||
| 9 | VAL | ASP | LEU | ALA | ARG | LYS | SER | SER | LYS | PRO | ||||
| 10 | ILE | ILE | THR | VAL | ARG | PRO | TYR | GLY | LEU | GLU | ||||
| 11 | ASN | VAL | PRO | PRO | GLU | LEU | GLU | ALA | VAL | SER | ||||
| 12 | SER | GLU | VAL | VAL | GLY | TRP | ASN | PRO | HIS | CYS | ||||
| 13 | ILE | ARG | ASP | ALA | LEU | GLU | ASP | ALA | LEU | ASP | ||||
| 14 | VAL | ILE |
Samples:
sample_1: HYPOTHETICAL PROTEIN MTH538, [U-13C; U-15N], 1.5 – 2.0 mM; salt 150 mM; phosphate buffer 25 mM; DTT 5 mM
sample_cond_1: pH: 7.4; temperature: 298 K; ionic strength: 150 mM
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 4D 13C-separated_NOESY | not available | not available | not available |
| 3D 15N-separated_NOESY | not available | not available | not available |
| HMQC-J | not available | not available | not available |
| 3D 13C,15N-simultaneous NOESY | not available | not available | not available |
| 1H-15N HSQC D2O exchange | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCACONNH | not available | not available | not available |
| CBCACOCAHA | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| HCC-TOCSY-NNH | not available | not available | not available |
| CCC-TOCSY-NNH | not available | not available | not available |
Software:
X-PLOR v3.1 - structure solution
FELIX v98 - processing
VNMR - collection
NMR spectrometers:
- Varian UNITY plus 500 MHz
- Varian Inova 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks