BMRB Entry 4789

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for TAZ2 domain of CBP
Published: 2000-10-23

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4789

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for TAZ2 domain of CBP

Deposition date:

2000-07-19

Original release date:

2000-10-23

Authors:

De Guzman, Roberto; Liu, Hong; Martinez-Yamout, Maria; Dyson, H.; Wright, Peter

Citation:

Citation: De Guzman, Roberto; Liu, Hong; Martinez-Yamout, Maria; Dyson, H.; Wright, Peter. "Solution Structure of the TAZ2(CH3) Domain of the Transcriptional Adaptor Protein CBP" J. Mol. Biol. 303, 243-253 (2000).

Assembly members:

Assembly members:
CBP TAZ2 domain, polymer, 87 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CBP TAZ2 domain: SPQESRRLSIQRCIQSLVHA CQCRNANCSLPSCQKMKRVV QHTKGCKRKTNGGCPVCKQL IALCCYHAKHCQENKCPVPF CLNIKHK

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	514
13C chemical shifts	354
15N chemical shifts	103

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TAZ2	1
2	ZN_2+	2

Entities:

Entity 1, TAZ2 87 residues - Formula weight is not available

1

SER

PRO

GLN

GLU

SER

ARG

LEU

SER

ILE

2

GLN

ARG

CYS

ILE

GLN

SER

LEU

VAL

HIS

ALA

3

CYS

GLN

CYS

ARG

ASN

ALA

ASN

CYS

SER

LEU

4

PRO

SER

CYS

GLN

LYS

MET

LYS

ARG

VAL

5

GLN

HIS

THR

LYS

GLY

CYS

LYS

ARG

LYS

THR

6

ASN

GLY

CYS

PRO

VAL

CYS

LYS

GLN

LEU

7

ILE

ALA

LEU

CYS

TYR

HIS

ALA

LYS

HIS

8

CYS

GLN

GLU

ASN

LYS

CYS

PRO

VAL

PRO

PHE

9

CYS

LEU

ASN

ILE

LYS

HIS

LYS

Entity 2, ZN_2+ - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: CBP TAZ2 domain, [U-15N], 1 – 1.5 mM; ZINC (II) ION mM

sample_2: CBP TAZ2 domain, [U-15N; U-13C], 1 – 1.5 mM; ZINC (II) ION mM

Ex-cond_1: pH: 5.8; temperature: 298 K; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
15N NOESY	not available	not available	Ex-cond_1
15N TOCSY	not available	not available	Ex-cond_1
13C NOESY	not available	not available	Ex-cond_1
HNCA	not available	not available	Ex-cond_1
HNCACB	not available	not available	Ex-cond_1
CBCACONH	not available	not available	Ex-cond_1
HCCONH	not available	not available	Ex-cond_1
CCONH	not available	not available	Ex-cond_1
HNHA	not available	not available	Ex-cond_1
HNHB	not available	not available	Ex-cond_1
HACAHB-COSY	not available	not available	Ex-cond_1
HNCO	not available	not available	Ex-cond_1

Software:

NMRVIEW v4.1 - data analysis

NMRpipe - data processing

NMRDraw - data processing

NMR spectrometers:

Bruker AMX 500 MHz
Bruker DMX 600 MHz
Bruker DRX 750 MHz

SWISS-PROT	P45481 Q92793
REF	NP_001088637 NP_004371 NP_001073315 NP_001082924 NP_001020603
PRF	1923401A
GenBank	AAC51770 AAH86282 AAC17736 AAC51331 AAB28651
EMBL	CAN87737 CAK11048 CAM56592 CAF96470 CAG06082
PDB	1F81
DBJ	BAE06125