BMRB Entry 4788

Name: 1H,15N, and 13C NMR resonance assignments for the Eps15 Homology domain Resp1
Published: 2000-12-18

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4788

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H,15N, and 13C NMR resonance assignments for the Eps15 Homology domain Resp1

Deposition date:

2000-07-19

Original release date:

2000-12-18

Authors:

Kim, Soyoun; Feig, Larry; Baleja, James

Citation:

Citation: Kim, Soyoun; Dubelman, Ari; Goonesekera, Sunali; Feig, Larry; Baleja, James. "Letter to the Editor: 1H, 15N, and 13C NMR resonance assignments for the Eps15 homology domain of Reps1" J. Biomol. NMR 18, 367-368 (2000).

Assembly members:

Assembly members:
Eps15 homology domain, polymer, 97 residues, 11093 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Eps15 homology domain: ASVGSWKITDEQRQYYVNQF KTIQPDLNGFIPGSAAKEFF TKSKLPILELSHIWELSDFD KDGALTLDEFCAAFHLVVAR KNGYDLPEKLPESLMPK

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	681
13C chemical shifts	286
15N chemical shifts	93

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Reps1 EH domain	1

Entities:

Entity 1, Reps1 EH domain 97 residues - 11093 Da.

1

ALA

SER

VAL

GLY

SER

TRP

LYS

ILE

THR

ASP

2

GLU

GLN

ARG

GLN

TYR

VAL

ASN

GLN

PHE

3

LYS

THR

ILE

GLN

PRO

ASP

LEU

ASN

GLY

PHE

4

ILE

PRO

GLY

SER

ALA

LYS

GLU

PHE

5

THR

LYS

SER

LYS

LEU

PRO

ILE

LEU

GLU

LEU

6

SER

HIS

ILE

TRP

GLU

LEU

SER

ASP

PHE

ASP

7

LYS

ASP

GLY

ALA

LEU

THR

LEU

ASP

GLU

PHE

8

CYS

ALA

PHE

HIS

LEU

VAL

ALA

ARG

9

LYS

ASN

GLY

TYR

ASP

LEU

PRO

GLU

LYS

LEU

10

PRO

GLU

SER

LEU

MET

PRO

LYS

Samples:

sample_1: Eps15 homology domain, [U-99% 13C; U-99% 15N], 1.3 mM

sample_2: Eps15 homology domain, [U-10% 13C; U-99% 15N], 1.0 mM

sample_3: Eps15 homology domain, [U-15N]-Leu, 0.5 mM

sample_4: Eps15 homology domain, [U-15N]-Phe, 0.5 mM

Ex-cond_1: pH: 6.8; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY	not available	not available	not available
1H-15N TOCSY	not available	not available	not available
HNCA	not available	not available	not available
HN(CO)CA	not available	not available	not available
HNCBCA	not available	not available	not available
HNCB(CO)CA	not available	not available	not available
HNHB	not available	not available	not available
1H-15N HSQC	not available	not available	not available
1H-13C HSQC	not available	not available	not available
CT 1H-13C HSQC experiments were used for stereospecific assignment	not available	not available	not available
of methyl protons of Val and Leu with 10% 13C and 99% 15N labeled sample.	not available	not available	not available
(sample_2)	not available	not available	not available

Software:

FELIX v4.2 -

NMR spectrometers:

Bruker AMX 500 MHz
Bruker Avance 600 MHz

PDB	1FI6
DBJ	BAC31117 BAE29439 BAE30291 BAG35780
EMBL	CAG03521 CAG31901 CDQ63378 CDQ64642
GB	AAB94736 AAH12764 AAH21211 AAH87547 AAI33364
REF	NP_001012895 NP_001099734 NP_001104535 NP_001122089 NP_001179940
SP	O54916 Q96D71
TPG	DAA26089
AlphaFold	O54916 Q96D71