BMRB Entry 4785

Title:
1H, 13C, and 15N assignment of a bleomycin resistance protein in its native form and in a complex with Zn(2+) ligated bleomycin
Deposition date:
2000-07-17
Original release date:
2000-10-30
Authors:
Vanbelle, Christophe; Muhle-Goll, Claudia; Remy, Marie-Helene; Masson, Jean-Michel; Marion, Dominique; Brutscher, Bernhard
Citation:

Citation: Vanbelle, Christophe; Muhle-Goll, Claudia; Remy, Marie-Helene; Masson, Jean-Michel; Marion, Dominique; Brutscher, Bernhard. "Letter to the Editor: 1H, 13C, and 15N assignment of a bleomycin resistance protein in its native form and in a complex with Zn2+ ligated bleomycin"  J. Biomol. NMR 18, 177-178 (2000).

Assembly members:

Assembly members:
S. hindustanus bleomycin resistance protein, polymer, 124 residues, 13796 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 2017   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptoalloteichus hindustanus

Experimental source:   Production method: recombinant technology

Experimental source:

Natural source:   Common Name: not available   Taxonomy ID: 2017   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptoalloteichus hindustanus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts115
13C chemical shifts228
15N chemical shifts115

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BRP monomer 11
2BRP monomer 21

Entities:

Entity 1, BRP monomer 1 124 residues - 13796 Da.

1   METALALYSLEUTHRSERALAVALPROVAL
2   LEUTHRALAARGASPVALALAGLYALAVAL
3   GLUPHETRPTHRASPARGLEUGLYPHESER
4   ARGASPPHEVALGLUASPASPPHEALAGLY
5   VALVALARGASPASPVALTHRLEUPHEILE
6   SERALAVALGLNASPGLNVALVALPROASP
7   ASNTHRLEUALATRPVALTRPVALARGGLY
8   LEUASPGLULEUTYRALAGLUTRPSERGLU
9   VALVALSERTHRASNPHEARGASPALASER
10   GLYPROALAMETTHRGLUILEGLYGLUGLN
11   PROTRPGLYARGGLUPHEALALEUARGASP
12   PROALAGLYASNCYSVALHISPHEVALALA
13   GLUGLUGLNASP

Samples:

sample_1: S. hindustanus bleomycin resistance protein, [U-99% 15N], 1.0 mM

sample_2: S. hindustanus bleomycin resistance protein, [U-98% 13C; U-99% 15N], 1 mM

Cond_1: pH*: 6.5; temperature: 313 K; ionic strength: 0.1 M

Experiments:

NameSampleSample stateSample conditions

Software:

FELIX v97 -

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 4786
PDB
DBJ BAC41730 BAF75469 BAG54978 BAG71041 BAI39424
EMBL CAA37050 CAA83658 CAD21454
GB AAA72449 AAA72451 AAA73162 AAA73163 AAA93507
SP P17493
AlphaFold P17493

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts