BMRB Entry 4782
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4782
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Citation: Legge, Glen; Poget, Sebastien; Proctor, Mark; Freund, Stefan; Bycroft, Mark. "Letter to the Editor:
1H, 13C and 15N NMR assignments of the C-type lectin TC14" J. Biomol. NMR 18, 283-284 (2000).
Assembly members:
Polyandrocarpa lectin, polymer, 125 residues, 14031 Da.
CA, non-polymer, 40.078 Da.
Natural source: Common Name: not available Taxonomy ID: 7723 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Polyandrocarpa misakiensis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Polyandrocarpa lectin: MDYEILFSDETMNYADAGTY
CQSRGMALVSSAMRDSTMVK
AILAFTEVKGHDYWVGADNL
QDGAYNFLWNDGVSLPTDSD
LWSPNEPSNPQSWQLCVQIW
SKYNLLDDVGCGGARRVICE
KELDD
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 669 |
| 13C chemical shifts | 404 |
| 15N chemical shifts | 125 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TC14 subunit 1 | 1 |
| 2 | TC14 subunit 2 | 1 |
| 3 | CA 1 | 2 |
| 4 | CA 2 | 2 |
Entities:
Entity 1, TC14 subunit 1 125 residues - 14031 Da.
| 1 | MET | ASP | TYR | GLU | ILE | LEU | PHE | SER | ASP | GLU | ||||
| 2 | THR | MET | ASN | TYR | ALA | ASP | ALA | GLY | THR | TYR | ||||
| 3 | CYS | GLN | SER | ARG | GLY | MET | ALA | LEU | VAL | SER | ||||
| 4 | SER | ALA | MET | ARG | ASP | SER | THR | MET | VAL | LYS | ||||
| 5 | ALA | ILE | LEU | ALA | PHE | THR | GLU | VAL | LYS | GLY | ||||
| 6 | HIS | ASP | TYR | TRP | VAL | GLY | ALA | ASP | ASN | LEU | ||||
| 7 | GLN | ASP | GLY | ALA | TYR | ASN | PHE | LEU | TRP | ASN | ||||
| 8 | ASP | GLY | VAL | SER | LEU | PRO | THR | ASP | SER | ASP | ||||
| 9 | LEU | TRP | SER | PRO | ASN | GLU | PRO | SER | ASN | PRO | ||||
| 10 | GLN | SER | TRP | GLN | LEU | CYS | VAL | GLN | ILE | TRP | ||||
| 11 | SER | LYS | TYR | ASN | LEU | LEU | ASP | ASP | VAL | GLY | ||||
| 12 | CYS | GLY | GLY | ALA | ARG | ARG | VAL | ILE | CYS | GLU | ||||
| 13 | LYS | GLU | LEU | ASP | ASP |
Entity 2, CA 1 - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: Polyandrocarpa lectin 1.5 mM
sample_2: Polyandrocarpa lectin, [U-15N], 1.5 mM
sample_3: Polyandrocarpa lectin, [U-13C; U-15N], 1.5 mM
Ex-cond_1: pH*: 6.0; temperature: 328 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| CBCA(CO)NH | not available | not available | Ex-cond_1 |
| HNCA | not available | not available | Ex-cond_1 |
| HNCACB | not available | not available | Ex-cond_1 |
| HBHA(CBCACO)NH | not available | not available | Ex-cond_1 |
| 1H-15N TOCSY-HMQC | not available | not available | Ex-cond_1 |
| 1H-15N HSQC | not available | not available | Ex-cond_1 |
| 1H-13C HSQC | not available | not available | Ex-cond_1 |
| 1H-1H DQF-COSY | not available | not available | Ex-cond_1 |
| 1H-1H TOCSY | not available | not available | Ex-cond_1 |
| 1H-13C HCCH TOCSY | not available | not available | Ex-cond_1 |
Software:
FELIX v2.30 -
NMR spectrometers:
- Bruker AMX 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks