BMRB Entry 4772

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4772

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for the N-terminal RNA-binding domain of human RNA-binding protein with multiple splicing
Deposition date:
2000-06-28
Original release date:
2000-09-14
Authors:
Sahasrabudhe, Parag; Montelione, Gaetano
Citation:

Citation: Sahasrabudhe, Parag; Xiao, R.; Montelione, Gaetano. "Letter to the Editor: Resonance assignments for the N-terminal domain from human RNA-binding protein with multiple splicing (RBP-MS)"  J. Biomol. NMR 19, 285-286 (2001).

Assembly members:

Assembly members:
RNA-binding protein with multiple splicing, polymer, 111 residues, 12931 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA-binding protein with multiple splicing: MNNGGKAEKENTPSEANLQE EEVRTLFVSGLPLDIKPREL YLLFRPFKGYEGSLIKLTSK QPVGFVSFDSRSEAEAAKNA LNGIRFDPEIPQTLRLEFAK ANTKMAKNKLV

Data sets:
Data typeCount
13C chemical shifts290
15N chemical shifts85
1H chemical shifts85

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RBPMS subunit 11
2RBPMS subunit 21
3RBPMS subunit 31

Entities:

Entity 1, RBPMS subunit 1 111 residues - 12931 Da.

1   METASNASNGLYGLYLYSALAGLULYSGLU
2   ASNTHRPROSERGLUALAASNLEUGLNGLU
3   GLUGLUVALARGTHRLEUPHEVALSERGLY
4   LEUPROLEUASPILELYSPROARGGLULEU
5   TYRLEULEUPHEARGPROPHELYSGLYTYR
6   GLUGLYSERLEUILELYSLEUTHRSERLYS
7   GLNPROVALGLYPHEVALSERPHEASPSER
8   ARGSERGLUALAGLUALAALALYSASNALA
9   LEUASNGLYILEARGPHEASPPROGLUILE
10   PROGLNTHRLEUARGLEUGLUPHEALALYS
11   ALAASNTHRLYSMETALALYSASNLYSLEU
12   VAL

Samples:

sample_1: RNA-binding protein with multiple splicing, [U-100% 15N], 1.2 mM; Sodium Phosphate 50 mM; Sodium Azide 0.5 mM

sample_2: RNA-binding protein with multiple splicing, [U-100% 15N; U-100% 13C], 1.8 mM; Sodium Phosphate 50 mM; Sodium Azide 0.5 mM

sample_3: RNA-binding protein with multiple splicing, [U-100% 15N; U-100% 13C; U-48% 2H], 1.8 mM; Sodium Phosphate 50 mM; Sodium Azide 0.5 mM

Sample_condition: pH: 6.0; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availablenot availableSample_condition
3D HNCOnot availablenot availableSample_condition
3D HN(CA)COnot availablenot availableSample_condition
3D HNCAnot availablenot availableSample_condition
3D HN(CO)CAnot availablenot availableSample_condition
3D CBCANHnot availablenot availableSample_condition
3D CBCA(CO)NHnot availablenot availableSample_condition
3D HCC(CO)NH TOCSYnot availablenot availableSample_condition
3D 15N edited NOESY HSQCnot availablenot availableSample_condition

Software:

VNMR v5.3 - Data acquisition and processing

SPARKY - Spectral analysis and peak picking

AutoAssign - Automated resonance assignment

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

DBJ BAA12225 BAA12226 BAA12227 BAA12228 BAA12229
GB AAD39515 AAH03608 AAH30397 AAH92476 AAI12765
REF NP_001008710 NP_001008711 NP_001008712 NP_001036139 NP_001036140
SP Q93062 Q9WVB0
TPG DAA14503
AlphaFold Q9WVB0 Q93062

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks