BMRB Entry 4768

Title:
Structure of parvulin hPar14
Deposition date:
2000-06-22
Original release date:
2000-12-07
Authors:
Sekerina, Elena; Rahfeld, Jens; Muller, Jonathan; Fanghanel, Jorg; Rascher, Christine; Fischer, Gunter; Bayer, Peter
Citation:

Citation: Sekerina, Elena; Rahfeld, Jens; Muller, Jonathan; Fanghanel, Jorg; Rascher, Christine; Fischer, Gunter; Bayer, Peter. "NMR Solution Structure of hPar14 Reveals Similarity to the Peptidyl Prolyl cis/tans Isomerase Domain of the Mitotic Regulator hPin1 but Indicates a Different Functionality of the Protein"  J. Mol. Biol. 301, 1003-1017 (2000).

Assembly members:

Assembly members:
human parvulin 14, polymer, 131 residues, 13809.9 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
1H chemical shifts365
13C chemical shifts179
15N chemical shifts92

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hpar141

Entities:

Entity 1, hpar14 131 residues - 13809.9 Da.

1   METPROPROLYSGLYLYSSERGLYSERGLY
2   LYSALAGLYLYSGLYGLYALAALASERGLY
3   SERASPSERALAASPLYSLYSALAGLNGLY
4   PROLYSGLYGLYGLYASNALAVALLYSVAL
5   ARGHISILELEUCYSGLULYSHISGLYLYS
6   ILEMETGLUALAMETGLULYSLEULYSSER
7   GLYMETARGPHEASNGLUVALALAALAGLN
8   TYRSERGLUASPLYSALAARGGLNGLYGLY
9   ASPLEUGLYTRPMETTHRARGGLYSERMET
10   VALGLYPROPHEGLNGLUALAALAPHEALA
11   LEUPROVALSERGLYMETASPLYSPROVAL
12   PHETHRASPPROPROVALLYSTHRLYSPHE
13   GLYTYRHISILEILEMETVALGLUGLYARG
14   LYS

Samples:

sample1: human parvulin 141.0 – 2.0 mM

sample2: human parvulin 14, [U-13C; U-15N], 1.0 – 2.0 mM

cond-1: pH: 6.5; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions

Software:

NDee - assignment of 2D spectra

Aurelia - assignment of 3D spectra

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ BAA82320 BAB26863 BAG54532
GB AAD27893 AAH05234 AAH70288 AAH93700 AAI04654
REF NP_001164218 NP_006214 NP_081457 XP_001092957 XP_001119408
SP Q9CWW6 Q9Y237
AlphaFold Q9CWW6 Q9Y237

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks