BMRB Entry 4761

Title:
Magnetic Susceptibility Tensor and Heme Contact Shifts Determinations in the Rhodobacter capsulatus Ferricytochrome c': NMR and Magnetic Susceptibility Studies
Deposition date:
2000-06-14
Original release date:
2001-08-09
Authors:
Tsan, Pascale; Caffrey, Michael; Lawson Daku, Max; Cusanovich, Michael; Marion, Dominique; Gans, Pierre
Citation:

Citation: Tsan, Pascale; Caffrey, Michael; Lawson Daku, Max; Cusanovich, Michael; Marion, Dominique; Gans, Pierre. "Magnetic Susceptibility Tensor and Heme Contact Shifts Determinations in the Rhodobacter capsulatus Ferricytochrome c': NMR and Magnetic Susceptibility Studies"  J. Am. Chem. Soc. 123, 2231-2242 (2001).

Assembly members:

Assembly members:
cytochrome c', polymer, 129 residues, Formula weight is not available
HEC, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 1061   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Rhodobacter capsulatus

Experimental source:

Experimental source:   Production method: purified from the natural source

Data sets:
Data typeCount
15N chemical shifts130
1H chemical shifts725

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apocytochrome c'1
2protoporphyrin IX, heme2

Entities:

Entity 1, apocytochrome c' 129 residues - Formula weight is not available

1   ALAASPTHRLYSGLUVALLEUGLUALAARG
2   GLUALATYRPHELYSSERLEUGLYGLYSER
3   METLYSALAMETTHRGLYVALALALYSALA
4   PHEASPALAGLUALAALALYSALAGLUALA
5   ALALYSLEUGLULYSILELEUALATHRASP
6   VALALAPROLEUPHEPROALAGLYTHRSER
7   SERTHRASPLEUPROGLYGLNTHRGLUALA
8   LYSALAALAILETRPALAASNMETASPASP
9   PHEGLYALALYSGLYLYSALAMETHISGLU
10   ALAGLYGLYALAVALILEALAALAALAASN
11   ALAGLYASPGLYALAALAPHEGLYALAALA
12   LEUGLNLYSLEUGLYGLYTHRCYSLYSALA
13   CYSHISASPASPTYRARGGLUGLUASP

Entity 2, protoporphyrin IX, heme - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_1: cytochrome c', [U-95 % 15N], 7 mM; potassium phosphate 100 mM; sodium hydrosulfite mM; carbon monoxide mM

cond_1: pH: .; temperature: . K

Experiments:

NameSampleSample stateSample conditions
not availablesample_1not availablecond_1

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
GB AAF37605 ADE86412 ETD00662 ETD75294 ETD82072
REF WP_013068391 WP_023921469 YP_003578819

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks