BMRB Entry 4739

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4739

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR solution structure of the calcium-bound C-terminal domain (W81-S161) of Calcium Vector Protein from Amphioxus

Deposition date:

2000-05-19

Original release date:

2000-12-18

Authors:

Theret, Isabelle; Baladi, Sybil; Cox, Jos; Sakamoto, Hiroshi; Craescu, Constantin

Citation:

Citation: Theret, Isabelle; Baladi, Sybil; Cox, Jos; Sakamoto, Hiroshi; Craescu, Constantin. "Sequential Calcium Binding to the Regulatory Domain of Calcium Vector Protein Reveals Functional Asymmetry and a Novel Mode of Structural Rearrangement" Biochemistry 39, 7920-7926 (2000).

Assembly members:

Assembly members:
Calcium Vector Protein, polymer, 81 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: amphioxus, lancelet Taxonomy ID: 7740 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Branchiostoma lanceolatum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Calcium Vector Protein: WVRQDDEEEILRAFKVFDAN GDGVIDFDEFKFIMQKVGEE PLTDAEVEEAMKEADEDGNG VIDIPEFMDLIKKSKNALKE S

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	560
15N chemical shifts	80

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CaVP	1
2	Ca 2+	2

Entities:

Entity 1, CaVP 81 residues - Formula weight is not available

1

TRP

VAL

ARG

GLN

ASP

GLU

ILE

2

LEU

ARG

ALA

PHE

LYS

VAL

PHE

ASP

ALA

ASN

3

GLY

ASP

GLY

VAL

ILE

ASP

PHE

ASP

GLU

PHE

4

LYS

PHE

ILE

MET

GLN

LYS

VAL

GLY

GLU

5

PRO

LEU

THR

ASP

ALA

GLU

VAL

GLU

ALA

6

MET

LYS

GLU

ALA

ASP

GLU

ASP

GLY

ASN

GLY

7

VAL

ILE

ASP

ILE

PRO

GLU

PHE

MET

ASP

LEU

8

ILE

LYS

SER

LYS

ASN

ALA

LEU

LYS

GLU

9

SER

Entity 2, Ca 2+ - Ca - 40.078 Da.

1

CA

Samples:

sample_1: Calcium Vector Protein0.8 – 1.2 mM; Tris buffer, [U-2H], 20 mM; KCl 100 mM

sample_2: Calcium Vector Protein, [U-15N], 1 mM; Tris buffer, [U-2H], 20 mM; KCl 100 mM

cond_1: pH: 6.5; temperature: 308 K; ionic strength: 0.100 M

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	not available	not available	cond_1
2D 1H-1H TOCSY	not available	not available	cond_1
2D 1H-1H DQF-COSY	not available	not available	cond_1
2D 1H-1H DQ-COSY	not available	not available	cond_1
2D 1H-15N HSQC	not available	not available	cond_1
3D 1H-1H-15N NOESY	not available	not available	cond_1
3D 1H-1H-15N TOCSY	not available	not available	cond_1

Software:

FELIX v97.0 - Spectra processing, peak-picking, restraint analysis

NMR spectrometers:

Varian Unity 500 MHz

PDB	1C7V 1C7W
DBJ	BAA19428 BAA19429 BAA74696 BAA74697 BAA96551
GB	AEJ84494 EEN54468
REF	XP_002598456
SP	O01305 P04573
AlphaFold	O01305 P04573