BMRB Entry 4719

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4719

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N chemical shift assignments of the Ras-binding domain (RBD) of AF6

Deposition date:

2000-04-12

Original release date:

2001-03-15

Authors:

Steiner, Guido; Kremer, Werner; Linnemann, Thomas; Herrmann, Christian; Geyer, Matthias; Kalbitzer, Hans

Citation:

Citation: Steiner, Guido; Kremer, Werner; Linnemann, Thomas; Herrmann, Christian; Geyer, Matthias; Kalbitzer, Hans. "Sequence-specific Resonance Assignment of the Ras-binding Domain of AF6" J. Biomol. NMR 18, 73-74 (2000).

Assembly members:

Assembly members:
rnAF6, polymer, 141 residues, 16481 Da.

Natural source:

Natural source: Common Name: rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norwegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
rnAF6: MSAGGRDEERRKLADIIHHW NANRLDLFEISQPTEDLEFH GVMRFYFQDKAAGNFATKCI RVSSTATTQDVIETLAEKFR PDMRMLSSPKYSLYEVHVSG ERRLDIDEKPLVVQLNWNKD DREGRFVLKNENDAIPAKKA Q

Data sets:

assigned_chemical_shifts
- H_HA_C_CA_CB_and_N_chemical_shifts_of_AF6

Data type	Count
13C chemical shifts	483
15N chemical shifts	124
1H chemical shifts	754

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AF6	1

Entities:

Entity 1, AF6 141 residues - 16481 Da.

1

MET

SER

ALA

GLY

ARG

ASP

GLU

ARG

2

ARG

LYS

LEU

ALA

ASP

ILE

HIS

TRP

3

ASN

ALA

ASN

ARG

LEU

ASP

LEU

PHE

GLU

ILE

4

SER

GLN

PRO

THR

GLU

ASP

LEU

GLU

PHE

HIS

5

GLY

VAL

MET

ARG

PHE

TYR

PHE

GLN

ASP

LYS

6

ALA

GLY

ASN

PHE

ALA

THR

LYS

CYS

ILE

7

ARG

VAL

SER

THR

ALA

THR

GLN

ASP

8

VAL

ILE

GLU

THR

LEU

ALA

GLU

LYS

PHE

ARG

9

PRO

ASP

MET

ARG

MET

LEU

SER

PRO

LYS

10

TYR

SER

LEU

TYR

GLU

VAL

HIS

VAL

SER

GLY

11

GLU

ARG

LEU

ASP

ILE

ASP

GLU

LYS

PRO

12

LEU

VAL

GLN

LEU

ASN

TRP

ASN

LYS

ASP

13

ASP

ARG

GLU

GLY

ARG

PHE

VAL

LEU

LYS

ASN

14

GLU

ASN

ASP

ALA

ILE

PRO

ALA

LYS

ALA

15

GLN

Samples:

unlabeled_protein: rnAF6 0.9 mM

15N_labeled_protein: rnAF6, [U-15N], 1.3 mM

13C_15N_labeled_protein: rnAF6, [U-13C; U-15N], 1.0 – 1.3 mM

standard_conditions: pH: 6.9; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	not available
HNCA	not available	not available	not available
HNCO	not available	not available	not available
HN(CO)CA	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
CBCANH	not available	not available	not available
15N-TOCSY-HSQC	not available	not available	not available
15N-NOESY-HSQC	not available	not available	not available
HCCH-TOCSY	not available	not available	not available
13C-NOESY-HSQC	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Bruker DMX 500 MHz
Bruker DRX 600 MHz
Bruker DRX 800 MHz

DBJ	BAA32483 BAA32484 BAA32485 BAD92657 BAE38754
GB	AAC50059 AAC53390 AAC53391 AAD54283 AAI56049
REF	NP_001035089 NP_001193937 NP_001278893 NP_034936 NP_037349
SP	O35889 P55196 Q9QZQ1
TPG	DAA25947
AlphaFold	O35889 P55196 Q9QZQ1