BMRB Entry 4716

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PDB ID: 1n4c
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4716
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N, and 13C NMR Backbone Assignments and Secondary Structure of the C-terminal Recombinant Fragment of Auxilin Including the J-domain

Deposition date:

2000-04-11

Original release date:

2000-07-24

Authors:

Han, Chae; Gruschus, James; Greener, Tsvika; Greene, Lois; Ferretti, James; Eisenberg, Evan

Citation:

Citation: Han, Chae; Gruschus, James; Greener, Tsvika; Greene, Lois; Ferretti, James; Eisenberg, Evan. "Letter to the Editor: 1H, 15N, and 13C NMR backbone assignments and secondary structure of the C-terminal recombinant fragment of auxilin including the J-domain" J. Biomol. NMR 17, 281-282 (2000).

Assembly members:

Assembly members:
auxilin, polymer, 182 residues, 20563 Da.

Natural source:

Natural source: Common Name: cow Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
auxilin: GPLGSPEFSMPHSSPQNRPN YNVSFSSMPGGQNERGKAAA NLEGKQKAADFEDLLSGQGF NAHKDKKGPRTIAEMRKEEM AKEMDPEKLKILEWIEGKER NIRALLSTMHTVLWAGETKW KPVGMADLVTPEQVKKVYRK AVLVVHPDKATGQPYEQYAK MIFMELNDAWSEFENQGQKP LY

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	651
13C chemical shifts	361
15N chemical shifts	154

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	auxilin	1

Entities:

Entity 1, auxilin 182 residues - 20563 Da.

1

GLY

PRO

LEU

GLY

SER

PRO

GLU

PHE

SER

MET

2

PRO

HIS

SER

PRO

GLN

ASN

ARG

PRO

ASN

3

TYR

ASN

VAL

SER

PHE

SER

MET

PRO

GLY

4

GLY

GLN

ASN

GLU

ARG

GLY

LYS

ALA

5

ASN

LEU

GLU

GLY

LYS

GLN

LYS

ALA

ASP

6

PHE

GLU

ASP

LEU

SER

GLY

GLN

GLY

PHE

7

ASN

ALA

HIS

LYS

ASP

LYS

GLY

PRO

ARG

8

THR

ILE

ALA

GLU

MET

ARG

LYS

GLU

MET

9

ALA

LYS

GLU

MET

ASP

PRO

GLU

LYS

LEU

LYS

10

ILE

LEU

GLU

TRP

ILE

GLU

GLY

LYS

GLU

ARG

11

ASN

ILE

ARG

ALA

LEU

SER

THR

MET

HIS

12

THR

VAL

LEU

TRP

ALA

GLY

GLU

THR

LYS

TRP

13

LYS

PRO

VAL

GLY

MET

ALA

ASP

LEU

VAL

THR

14

PRO

GLU

GLN

VAL

LYS

VAL

TYR

ARG

LYS

15

ALA

VAL

LEU

VAL

HIS

PRO

ASP

LYS

ALA

16

THR

GLY

GLN

PRO

TYR

GLU

GLN

TYR

ALA

LYS

17

MET

ILE

PHE

MET

GLU

LEU

ASN

ASP

ALA

TRP

18

SER

GLU

PHE

GLU

ASN

GLN

GLY

GLN

LYS

PRO

19

LEU

TYR

Samples:

auxilin_1: auxilin1.0 – 2.0 mM

condition_1: pH: 7.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
15N-HSQC NOESY	not available	not available	not available
15N-HSQC TOCSY	not available	not available	not available
CBCACONH	not available	not available	not available
CBCANH	not available	not available	not available

Software:

NMRPipe -

NMR spectrometers:

Bruker DRX 600 MHz

PDB	1N4C 1XI5 2QWN 2QWO 2QWP 2QWQ 2QWR
DBJ	BAA32318 BAG11195 BAH12344
GB	AAA79037 AAI09280 AAI09281 AIC59549 EAX06533
REF	NP_001243793 NP_001243794 NP_055602 NP_777261 XP_001089936
SP	O75061 Q27974
TPG	DAA31275
AlphaFold	O75061 Q27974