BMRB Entry 4712

Name: 1H, 15N and 13C Assignments and secondary structure of nFGF
Published: 2000-07-24

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4712

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C Assignments and secondary structure of nFGF

Deposition date:

2000-04-10

Original release date:

2000-07-24

Authors:

Alphonse, Arunkumar; Thallampuranam, Suresh Kumar; Sampath, Srisailam; Kathir, K.; Yu, Chin

Citation:

Citation: Arunkumar, Alphonse; Srisailam, Sampath; Kumar, Thallampuranam; Kathir, K.; Peng, C.; Chen, C.; Chiu, I.; Yu, Chin. "Letter to the Editor: 1H, 13C and 15N chemical shift assignments of the acidic fibroblast growth factor from Notopthalmus viridescens" J. Biomol. NMR 17, 279-280 (2000).

Assembly members:

Assembly members:
newt acidic FGF, polymer, 132 residues, 15021 Da.
SUCROSE OCTASULFATE, non-polymer, 982.802 Da.

Natural source:

Natural source: Common Name: newt Taxonomy ID: 8316 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Notopthalmus viridescens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
newt acidic FGF: QKPKLLYCSNGGYFLRIFPD GKVDGTRDRSDPYIQLQFYA ESVGEVYIKSLETGQYLAMD SDGQLYASQSPSEECLFLER LEENNYNTYKSKVHADKDWF VGIKKNGKTKPGSRTHFGQK AILFLPLPVSSD

Data sets:

assigned_chemical_shifts
- nFGF-1A

Data type	Count
13C chemical shifts	530
15N chemical shifts	132
1H chemical shifts	783

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	nFGF	1
2	sucrose octasulfate	2

Entities:

Entity 1, nFGF 132 residues - 15021 Da.

1

GLN

LYS

PRO

LYS

LEU

TYR

CYS

SER

ASN

2

GLY

TYR

PHE

LEU

ARG

ILE

PHE

PRO

ASP

3

GLY

LYS

VAL

ASP

GLY

THR

ARG

ASP

ARG

SER

4

ASP

PRO

TYR

ILE

GLN

LEU

GLN

PHE

TYR

ALA

5

GLU

SER

VAL

GLY

GLU

VAL

TYR

ILE

LYS

SER

6

LEU

GLU

THR

GLY

GLN

TYR

LEU

ALA

MET

ASP

7

SER

ASP

GLY

GLN

LEU

TYR

ALA

SER

GLN

SER

8

PRO

SER

GLU

CYS

LEU

PHE

LEU

GLU

ARG

9

LEU

GLU

ASN

TYR

ASN

THR

TYR

LYS

10

SER

LYS

VAL

HIS

ALA

ASP

LYS

ASP

TRP

PHE

11

VAL

GLY

ILE

LYS

ASN

GLY

LYS

THR

LYS

12

PRO

GLY

SER

ARG

THR

HIS

PHE

GLY

GLN

LYS

13

ALA

ILE

LEU

PHE

LEU

PRO

LEU

PRO

VAL

SER

14

SER

ASP

Entity 2, sucrose octasulfate - C12 H22 O35 S8 - 982.802 Da.

1

SCR

Samples:

sample_1: newt acidic FGF, [U-95% 13C; U-90% 15N], 1.5 – 2 mM; SUCROSE OCTASULFATE1.5 – 2 mM

Ex-cond: pH: 6.1; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
15N-EDITED TOCSY	sample_1	not available	Ex-cond
15N-EDITED NOESY	sample_1	not available	Ex-cond
HNCA	sample_1	not available	Ex-cond
HN(CO)CA	sample_1	not available	Ex-cond
13C-EDITED NOESY	sample_1	not available	Ex-cond
HC-CH TOCSY	sample_1	not available	Ex-cond
HNCO	sample_1	not available	Ex-cond
C(CO)NH	sample_1	not available	Ex-cond
HCC0NH	sample_1	not available	Ex-cond
CBCBCONH	sample_1	not available	Ex-cond
CBCANH	sample_1	not available	Ex-cond
HNHA	sample_1	not available	Ex-cond

Software:

XWINNMR-AURELIA v2.1/2.1.5 -

NMR spectrometers:

Bruker DMX 600 MHz

PDB	1FMM
SP	Q7SIF8
AlphaFold	Q7SIF8